Abstract
Alteration of the Developing Testis Transcriptome Following Embryonic Vinclozolin Exposure
Biology of reproduction, Vol.81(Suppl_1), pp.644-644
07/01/2009
Abstract
The current study investigates the direct effects of in utero vinclozolin exposure on the developing rat testis transcriptome. Vinclozolin is a commonly used fungicide in agriculture and is an endocrine disruptor with anti-androgenic activity. Previous studies have demonstrated that exposure to vinclozolin during embryonic gonadal sex determination induces epigenetic modifications of the germ line and transgenerational adult onset disease states that include spermatogenic cell defects, prostate disease, kidney disease, and tumor development. An investigation of the molecular actions of vinclozolin was initiated through an analysis of direct actions on the F1 generation embryonic testis development. Microarray analyses were performed to compare control and vinclozolin treated testis transcriptomes at embryonic day 13, 14 and 16. A total of 567 differentially expressed genes were identified and the major cellular functions and pathways associated with these altered transcripts were examined. The sets of regulated genes at the different development periods were found to be transiently altered and distinct. Interestingly, genes previously shown to be regulated during normal male sex determination were not altered by vinclozolin treatment. Categorization by major known functions of all 576 genes altered by in utero vinclozolin exposure demonstrates transcription, signaling, cytoskeletal and extra cellular matrix associated transcripts are highly represented. Specific cellular process and pathway analysis suggests the involvement of Wnt and calcium signaling, vascular development and epigenetic mechanisms as potential mediators of the direct actions of vinclozolin.
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Details
- Title
- Alteration of the Developing Testis Transcriptome Following Embryonic Vinclozolin Exposure
- Creators
- Tracy Clement - Washington State UniversityMarina I. SavenkovaMathew SettlesMathew Anway - University of IdahoMichael Skinner
- Publication Details
- Biology of reproduction, Vol.81(Suppl_1), pp.644-644
- Academic Unit
- Biological Sciences, School of
- Identifiers
- 99901080309501842
- Language
- English
- Resource Type
- Abstract