Accepted manuscript
3-D printed adjustable microelectrode arrays for electrochemical sensing and biosensing
Sensors and actuators. B, Chemical, Vol.230, pp.600-606
07/2016
Handle:
https://hdl.handle.net/2376/113546
PMCID: PMC4802967
PMID: 27019550
Abstract
•Silver microelectrode arrays (MEA) were prepared using aerosol jet technology.•Silver trace spacing of the MEAs is controlled to be 30, 100 and 180μm, respectively.•Current density of MEA30:100:180 was 0.25:1:1, show diffusion layer overlap of MEA30.•MEA100 has the lowest detection limit towards H2O2, which was 0.45μM.
Printed electronics has emerged as an important fabrication technique that overcomes several shortcomings of conventional lithography and provides custom rapid prototyping for various sensor applications. In this work, silver microelectrode arrays (MEA) with three different electrode spacing were fabricated using 3-D printing by the aerosol jet technology. The microelectrodes were printed at a length scale of about 15μm, with the space between the electrodes accurately controlled to about 2 times (30μm, MEA30), 6.6 times (100μm, MEA100) and 12 times (180μm, MEA180) the trace width, respectively. Hydrogen peroxide and glucose were chosen as model analytes to demonstrate the performance of the MEA for sensor applications. The electrodes are shown to reduce hydrogen peroxide with a reduction current proportional to the concentration of hydrogen peroxide for certain concentration ranges. Further, the sensitivity of the current for the three electrode configurations was shown to decrease with an increase in the microelectrode spacing (sensitivity of MEA30:MEA100:MEA180 was in the ratio of 3.7:2.8:1), demonstrating optimal MEA geometry for such applications. The noise of the different electrode configurations is also characterized and shows a dramatic reduction from MEA30 to MEA100 and MEA180 electrodes. Further, it is shown that the response current is proportional to MEA100 and MEA180 electrode areas, but not for the area of MEA30 electrode (the current density of MEA30:MEA100:MEA180 is 0.25:1:1), indicating that the MEA30 electrodes suffer from diffusion overlap from neighboring electrodes. The work thus establishes the lower limit of microelectrode spacing for our geometry. The lowest detection limit of the MEAs was calculated (with S/N=3) to be 0.45μM. Glucose oxidase was immobilized on MEA100 microelectrodes to demonstrate a glucose biosensor application. The sensitivity of glucose biosensor was 1.73μAmM−1 and the calculated value of detection limit (S/N=3) was 1.7μM. The electrochemical response characteristics of the MEAs were in agreement with the predictions of existing models. The current work opens up the possibility of additive manufacturing as a fabrication technique for low cost custom-shaped MEA structures that can be used as electrochemical platforms for a wide range of sensor applications.
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Details
- Title
- 3-D printed adjustable microelectrode arrays for electrochemical sensing and biosensing
- Creators
- Haipeng Yang - School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, United StatesMd Taibur Rahman - School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, United StatesDan Du - School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, United StatesRahul Panat - School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, United StatesYuehe Lin - School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, United States
- Publication Details
- Sensors and actuators. B, Chemical, Vol.230, pp.600-606
- Academic Unit
- Mechanical and Materials Engineering, School of
- Publisher
- Elsevier B.V
- Number of pages
- 7
- Grant note
- RP's startup fund R210H010768 / Centers for Disease Control and Prevention/National Institute for Occupational Safety and Health (CDC/NIOSH); United States Department of Health & Human Services; Centers for Disease Control & Prevention - USA; National Institute for Occupational Safety & Health (NIOSH) JCYJ20150324140036855 / Shenzhen Science and Technology Research Grant 2015A030313545 / Guangdong Natural Science Foundation; National Natural Science Foundation of Guangdong Province R21OH010768 / NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH; United States Department of Health & Human Services; Centers for Disease Control & Prevention - USA; National Institute for Occupational Safety & Health (NIOSH)
- Identifiers
- 99900548199701842
- Language
- English
- Resource Type
- Accepted manuscript