A MAP kinase kinase interacts with SymRK and regulates nodule organogenesis in Lotus japonicus

Tao Chen; Hui Zhu; Danxia Ke; Kai Cai; Chao Wang; Honglan Gou; Zonglie Hong; Zhongming Zhang

doi:10.1105/tpc.112.095984

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A MAP kinase kinase interacts with SymRK and regulates nodule organogenesis in Lotus japonicus

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A MAP kinase kinase interacts with SymRK and regulates nodule organogenesis in Lotus japonicus

Tao Chen, Hui Zhu, Danxia Ke, Kai Cai, Chao Wang, Honglan Gou, Zonglie Hong and Zhongming Zhang

The Plant cell, Vol.24(2), pp.823-838

02/2012

DOI: https://doi.org/10.1105/tpc.112.095984

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https://hdl.handle.net/2376/104032

PMCID: PMC3315249

PMID: 22353370

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Abstract

Mitogen-Activated Protein Kinase Kinases - genetics

Arabidopsis - enzymology

Plants, Genetically Modified - growth & development

Arabidopsis - growth & development

Molecular Sequence Data

Gene Knockdown Techniques

Mitogen-Activated Protein Kinase Kinases - metabolism

Plant Root Nodulation - genetics

RNA Interference

Gene Expression Regulation, Plant

Loteae - growth & development

Plant Proteins - metabolism

Root Nodules, Plant - growth & development

Amino Acid Sequence

Plants, Genetically Modified - genetics

Plants, Genetically Modified - enzymology

Tobacco - growth & development

Arabidopsis - genetics

Plant Proteins - genetics

Tobacco - genetics

Loteae - enzymology

Tobacco - enzymology

Loteae - genetics

Phosphorylation

Signal Transduction

Symbiosis

The symbiosis receptor kinase, SymRK, is required for root nodule development. A SymRK-interacting protein (SIP2) was found to form protein complex with SymRK in vitro and in planta. The interaction between SymRK and SIP2 is conserved in legumes. The SIP2 gene was expressed in all Lotus japonicus tissues examined. SIP2 represents a typical plant mitogen-activated protein kinase kinase (MAPKK) and exhibited autophosphorylation and transphosphorylation activities. Recombinant SIP2 protein could phosphorylate casein and the Arabidopsis thaliana MAP kinase MPK6. SymRK and SIP2 could not use one another as a substrate for phosphorylation. Instead, SymRK acted as an inhibitor of SIP2 kinase when MPK6 was used as a substrate, suggesting that SymRK may serve as a negative regulator of the SIP2 signaling pathway. Knockdown expression of SIP2 via RNA interference (RNAi) resulted in drastic reduction of nodules formed in transgenic hairy roots. A significant portion of SIP2 RNAi hairy roots failed to form a nodule. In these roots, the expression levels of SIP2 and three marker genes for infection thread and nodule primordium formation were downregulated drastically, while the expression of two other MAPKK genes were not altered. These observations demonstrate an essential role of SIP2 in the early symbiosis signaling and nodule organogenesis.

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Title: A MAP kinase kinase interacts with SymRK and regulates nodule organogenesis in Lotus japonicus
Creators: Tao Chen - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
Hui Zhu
Danxia Ke
Kai Cai
Chao Wang
Honglan Gou
Zonglie Hong
Zhongming Zhang
Publication Details: The Plant cell, Vol.24(2), pp.823-838
Academic Unit: Center for Reproductive Biology; Department of Crop and Soil Sciences
Publisher: United States
Identifiers: 99900546540701842
Language: English
Resource Type: Accepted manuscript