Accepted manuscript
A Prolonged Nitric Oxide-Dependent, Opioid-Mediated Antinociceptive Effect of Hyperbaric Oxygen in Mice
The journal of pain, Vol.10(2), pp.167-172
2009
Handle:
https://hdl.handle.net/2376/109501
PMID: 18976963
Abstract
Hyperbaric oxygen (HBO 2) therapy is reported to cause pain relief in several conditions of chronic pain. A single 60-minute session of HBO 2 treatment produced a prolonged antinociceptive effect in mice that persisted for 90 minutes after cessation of treatment. The HBO 2 -induced antinociception was significantly attenuated by pretreatment before HBO 2 exposure with the opioid antagonist naltrexone, the nonspecific nitric oxide synthase (NOS)-inhibitor N G -nitro- l-arginine methyl ester (L-NAME), and the selective neuronal NOS-inhibitor S-methyl- l-thiocitrulline (SMTC) but not the selective endothelial NOS-inhibitor N 5-(1-iminoethyl)- l-ornithine (L-NIO). The antinociception was also significantly reduced by central pretreatment with a rabbit antiserum against dynorphin 1-13 but not by rabbit antisera against either β-endorphin or methionine-enkephalin. The prolonged antinociceptive effect at 90 minutes after HBO 2-induced treatment was also significantly attenuated by naltrexone but not L-NAME administered 60 minutes after HBO 2 treatment but before nociceptive testing. These findings indicate that the antinociception that persists for 90 minutes after HBO 2 exposure is mediated by nitric oxide (NO) and opioid mechanisms but that the NO involvement is critical during the HBO 2 treatment and not at the time of nociceptive testing. These results are consistent with the concept that HBO 2 may induce an NO-dependent release of opioid peptide to cause a long-acting antinociceptive effect. This article presents evidence of a persistent antinociceptive effect of hyperbaric oxygen treatment that is mediated by opioid and NO mechanisms. Further elucidation of the underlying mechanism could identify molecular targets to cause a longer-acting activation of endogenous pain-modulating systems.
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Details
- Title
- A Prolonged Nitric Oxide-Dependent, Opioid-Mediated Antinociceptive Effect of Hyperbaric Oxygen in Mice
- Creators
- Lisa M Zelinski - Department of Pharmaceutical Sciences, College of Pharmacy, Pullman, WashingtonYusuke Ohgami - Department of Pharmaceutical Sciences, College of Pharmacy, Pullman, WashingtonEunhee Chung - Department of Pharmaceutical Sciences, College of Pharmacy, Pullman, WashingtonDonald Y Shirachi - Chico Hyperbaric Center, Chico, CaliforniaRaymond M Quock - Department of Pharmaceutical Sciences, College of Pharmacy, Pullman, Washington
- Publication Details
- The journal of pain, Vol.10(2), pp.167-172
- Academic Unit
- Psychology, Department of
- Publisher
- Elsevier Inc
- Number of pages
- 6
- Identifiers
- 99900547205801842
- Language
- English
- Resource Type
- Accepted manuscript