Accepted manuscript
A Study of the Coriolis Effect on the Fluid Flow Profile in a Centrifugal Bioreactor
Biotechnology progress, Vol.25(4), pp.1025-1034
2009
Handle:
https://hdl.handle.net/2376/102011
PMCID: PMC3236108
PMID: 19455639
Abstract
Increasing demand for tissues, proteins, and antibodies derived from cell culture is necessitating the development and implementation of high cell density bioreactors. A system for studying high density culture is the centrifugal bioreactor (CCBR), which retains cells by increasing settling velocities through system rotation, thereby eliminating diffusional limitations associated with mechanical cell retention devices. This article focuses on the fluid mechanics of the CCBR system by considering Coriolis effects. Such considerations for centrifugal bioprocessing have heretofore been ignored; therefore, a simpler analysis of an empty chamber will be performed. Comparisons are made between numerical simulations and bromophenol blue dye injection experiments. For the non-rotating bioreactor with an inlet velocity of 4.3 cm/s, both the numerical and experimental results show the formation of a teardrop shaped plume of dye following streamlines through the reactor. However, as the reactor is rotated, the simulation predicts the development of vortices and a flow profile dominated by Coriolis forces resulting in the majority of flow up the leading wall of the reactor as dye initially enters the chamber, results are confirmed by experimental observations. As the reactor continues to fill with dye, the simulation predicts dye movement up both walls while experimental observations show the reactor fills with dye from the exit to the inlet. Differences between the simulation and experimental observations can be explained by excessive diffusion required for simulation convergence, and a slight density difference between dyed and un-dyed solutions. Implications of the results on practical bioreactor use are also discussed. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 1025-1034, 2009
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Details
- Title
- A Study of the Coriolis Effect on the Fluid Flow Profile in a Centrifugal Bioreactor
- Creators
- Christopher J DETZEL - Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710, United StatesMichael R THORSON - Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710, United StatesBernard J VAN WIE - Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710, United StatesCornelius F IVORY - Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710, United States
- Publication Details
- Biotechnology progress, Vol.25(4), pp.1025-1034
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Wiley; Hoboken, NJ
- Number of pages
- 10
- Grant note
- T32 GM008336-20; T32 GM008336; T32 GM008336-18; T32 GM008336-19; 5T32-GM008336-16 / NIGMS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) T32GM008336 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Identifiers
- 99900546791901842
- Language
- English
- Resource Type
- Accepted manuscript