Accepted manuscript
A bipolar kinesin
Nature (London), Vol.379(6562), pp.270-272
01/18/1996
Handle:
https://hdl.handle.net/2376/117052
PMCID: PMC3203953
PMID: 8538794
Abstract
Chromosome segregation during mitosis depends on the action of the mitotic spindle, a self-organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors 1 , 2 . Members of the BimC subfamily of kinesin-related MT–motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle 3 – 14 . Here we report that KRP 130 , a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos 13 , has an unusual ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament 15 . Such a bipolar ‘minifilament’ could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.
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Details
- Title
- A bipolar kinesin
- Creators
- A. S Kashina - Department of Biology, Indiana University, Bloomington, Indiana 47405, USAR. J Baskin - Department of Biology, Indiana University, Bloomington, Indiana 47405, USAD. G Cole - Department of Biology, Indiana University, Bloomington, Indiana 47405, USAK. P Wedaman - Department of Biology, Indiana University, Bloomington, Indiana 47405, USAW. M Saxton - Department of Biology, Indiana University, Bloomington, Indiana 47405, USAJ. M Scholey - Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
- Publication Details
- Nature (London), Vol.379(6562), pp.270-272
- Academic Unit
- Center for Reproductive Biology
- Number of pages
- 3
- Grant note
- R01 GM046295-09 || GM / National Institute of General Medical Sciences : NIGMS
- Identifiers
- 99900547849701842
- Language
- English
- Resource Type
- Accepted manuscript