Accepted manuscript
A new era for electron bifurcation
Current opinion in chemical biology, Vol.47(C), pp.32-38
12/2018
Handle:
https://hdl.handle.net/2376/103583
PMID: 30077080
Abstract
•Electron bifurcation couples endergonic and exergonic electron transfer reactions.•Electron bifurcation requires redox sites capable of managing single and pairwise electron transfer reactions.•Redox centers in electron bifurcating enzymes exist with an inherently short-lived low-potential redox state.•Crossed potential redox transitions have been observed at electron bifurcating sites, but are not required for electron bifurcation.•Electron bifurcation catalysis can occur at organic and potentially at inorganic cofactors that undergo at least two redox transitions.
Simple diagram of electron bifurcation illustrating the bifurcation of an electron pair from the electron donor (D) and the subsequent endergonic and exergonic electron transfers acceptors A1 and A2 respectively.▪
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Electron bifurcation, or the coupling of exergonic and endergonic oxidation-reduction reactions, was discovered by Peter Mitchell and provides an elegant mechanism to rationalize and understand the logic that underpins the Q cycle of the respiratory chain. Thought to be a unique reaction of respiratory complex III for nearly 40 years, about a decade ago Wolfgang Buckel and Rudolf Thauer discovered that flavin-based electron bifurcation is also an important component of anaerobic microbial metabolism. Their discovery spawned a surge of research activity, providing a basis to understand flavin-based bifurcation, forging fundamental parallels with Mitchell’s Q cycle and leading to the proposal of metal-based bifurcating enzymes. New insights into the mechanism of electron bifurcation provide a foundation to establish the unifying principles and essential elements of this fascinating biochemical phenomenon.
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Details
- Title
- A new era for electron bifurcation
- Creators
- John W Peters - Institute of Biological Chemistry, Washington State University, Pullman WA 99163, United StatesDavid N Beratan - Department of Chemistry and Department of Physics, Duke University, Durham, NC 27708, United StatesBrian Bothner - Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, United StatesR Brian Dyer - Department of Chemistry, Emory University, Atlanta, GA 30322, United StatesCaroline S Harwood - Department of Microbiology, University of Washington, Seattle, WA 98195, United StatesZachariah M Heiden - Department of Chemistry, Washington State University, Pullman WA 99163, United StatesRuss Hille - Biochemistry Department, University of California at Riverside, Riverside, CA 92521, United StatesAnne K Jones - School of Molecular Sciences, Arizona State University, Tempe, AZ 85287, United StatesPaul W King - National Renewable Energy Laboratory, Golden, CO 8040, United StatesYi Lu - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesCarolyn E Lubner - National Renewable Energy Laboratory, Golden, CO 8040, United StatesShelley D Minteer - Department of Chemistry, University of Utah, Salt Lake City, UT 84112, United StatesDavid W Mulder - National Renewable Energy Laboratory, Golden, CO 8040, United StatesSimone Raugei - Institute of Biological Chemistry, Washington State University, Pullman WA 99163, United StatesGerrit J Schut - Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA 30602, United StatesLance C Seefeldt - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesMonika Tokmina-Lukaszewska - Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, United StatesOleg A Zadvornyy - Institute of Biological Chemistry, Washington State University, Pullman WA 99163, United StatesPeng Zhang - Department of Biochemistry, Duke University, Durham, NC 27710, United StatesMichael WW Adams - Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA 30602, United States
- Publication Details
- Current opinion in chemical biology, Vol.47(C), pp.32-38
- Academic Unit
- Department of Chemistry; Institute of Biological Chemistry
- Publisher
- Elsevier Ltd
- Grant note
- R01 GM135088 / NIGMS NIH HHS
- Identifiers
- 99900546664101842
- Language
- English
- Resource Type
- Accepted manuscript