Alterations in the developing testis transcriptome following embryonic vinclozolin exposure

Tracy M CLEMENT; Marina I SAVENKOVA; Matthew SETTLES; Matthew D ANWAY; Michael K SKINNER

doi:10.1016/j.reprotox.2010.05.086

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Alterations in the developing testis transcriptome following embryonic vinclozolin exposure

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Alterations in the developing testis transcriptome following embryonic vinclozolin exposure

Tracy M CLEMENT, Marina I SAVENKOVA, Matthew SETTLES, Matthew D ANWAY and Michael K SKINNER

Reproductive toxicology (Elmsford, N.Y.), Vol.30(3), pp.353-364

2010

DOI: https://doi.org/10.1016/j.reprotox.2010.05.086

Handle:

https://hdl.handle.net/2376/111978

PMCID: PMC2944409

PMID: 20566332

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Abstract

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Medical sciences

Embryology: invertebrates and vertebrates. Teratology

Teratology. Teratogens

Toxicology

The current study investigates the direct effects of in utero vinclozolin exposure on the developing F1 generation rat testis transcriptome. Previous studies have demonstrated that exposure to vinclozolin during embryonic gonadal sex determination induces epigenetic modifications of the germ line and transgenerational adult onset disease states. Microarray analyses were performed to compare control and vinclozolin treated testis transcriptomes at embryonic days 13,14 and 16. A total of 576 differentially expressed genes were identified and the major cellular functions and pathways associated with these altered transcripts were examined. The sets of regulated genes at the different development periods were found to be transiently altered and distinct. Categorization by major known functions of altered genes was performed. Specific cellular process and pathway analyses suggest the involvement of Wnt and calcium signaling, vascular development and epigenetic mechanisms as potential mediators of the direct F1 generation actions of vinclozolin. (C) 2010 Elsevier Inc. All rights reserved.

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Title: Alterations in the developing testis transcriptome following embryonic vinclozolin exposure
Creators: Tracy M CLEMENT - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4236, United States
Marina I SAVENKOVA - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4236, United States
Matthew SETTLES - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4236, United States
Matthew D ANWAY - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4236, United States
Michael K SKINNER - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4236, United States
Publication Details: Reproductive toxicology (Elmsford, N.Y.), Vol.30(3), pp.353-364
Academic Unit: Biological Sciences, School of
Publisher: Elsevier; Amsterdam
Number of pages: 12
Grant note: NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
Identifiers: 99900547441901842
Language: English
Resource Type: Accepted manuscript