Accepted manuscript
Foldamers with hybrid biological and synthetic sequences as selective DNA fluorescent probes
Biochemistry (Easton), Vol.44(32), pp.10751-10756
08/16/2005
Handle:
https://hdl.handle.net/2376/108304
PMCID: PMC2516345
PMID: 16086577
Abstract
Foldable polymers with alternating single-strand deoxyribonucleic acid (ssDNA) and planar fluorescent organic chromophores can self-organize into folded nanostructures and hence are hybrid foldamers with biological sequences and synthetic properties. The biological sequence provides highly specific molecular recognition properties, while the physical properties of synthetic chromophores offer sensitive fluorescence detection. In this paper, we describe that rational designed hybrid foldamers exhibit potential in the detection of polynucleotides. Under strictly controlled laboratory conditions, fluorescence measurements indicate that configuration change due to binding of polynucleotides with one or two mismatched bases can be readily distinguished. These results shed light on the design and construction of nanostructured foldamers with actuator and sensory properties, which may find important applications as biological probes.
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Details
- Title
- Foldamers with hybrid biological and synthetic sequences as selective DNA fluorescent probes
- Creators
- Wei Wang - Washington State University, School of Chemical Engineering and BioengineeringWei WanAndrew StachiwAlexander D Q Li
- Publication Details
- Biochemistry (Easton), Vol.44(32), pp.10751-10756
- Academic Unit
- Department of Chemistry
- Publisher
- United States
- Grant note
- R01 GM065306-04 / NIGMS NIH HHS R01 GM065306-05 / NIGMS NIH HHS R01 GM065306-02 / NIGMS NIH HHS R01 GM065306-01A1 / NIGMS NIH HHS 065306 / PHS HHS R01 GM065306-03 / NIGMS NIH HHS
- Identifiers
- 99900547421901842
- Language
- English
- Resource Type
- Accepted manuscript