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Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing: Methods and Protocols
Accepted manuscript   Open access

Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing: Methods and Protocols

Millissia Ben Maamar, Ingrid Sadler-Riggleman, Daniel Beck and Michael K. Skinner
DNA Modifications , pp.301-310
Methods in Molecular Biology
01/01/2021
PMID: 32822040
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Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing305.26 kBDownloadView
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Abstract

Biochemical Research Methods Biochemistry & Molecular Biology Genetics & Heredity Life Sciences & Biomedicine Science & Technology
Methylated DNA immunoprecipitation is a large scale purification technique. It enables the isolation of methylated DNA fragments for subsequent locus-specific or genome-wide analysis. Here we describe an immunoprecipitation protocol using a monoclonal mouse anti 5-methyl-cytidine antibody followed by next-generation sequencing (MeDIP-Seq).

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