Accepted manuscript
Identification, characterization and field testing of Brassica napus mutants producing high-oleic oils
The Plant journal : for cell and molecular biology, Vol.98(1), pp.33-41
04/2019
PMID: 30536486
Abstract
Producing healthy, high-oleic oils and eliminating trans-fatty acids from foods are two goals that can be addressed by reducing activity of the oleate desaturase, FAD2, in oilseeds. However, it is essential to understand the consequences of reducing FAD2 activity on the metabolism, cell biology and physiology of oilseed crop plants. Here, we translate knowledge from studies of fad2 mutants in Arabidopsis (Arabidopsis thaliana) to investigate the limits of non-GMO approaches to maximize oleic acid in the seed oil of canola (Brassica napus), a species that expresses three active FAD2 isozymes. A series of hypomorphic and null mutations in the FAD2.A5 isoform were characterized in yeast (Saccharomyes cerevisiae). Then, four of these were combined with null mutations in the other two isozymes, FAD2.C5 and FAD2.C1. The resulting mutant lines contained 71-87% oleic acid in their seed oil, compared with 62% in wild-type controls. All the mutant lines grew well in a greenhouse, but in field experiments we observed a clear demarcation in plant performance. Mutant lines containing less than 80% oleate in the seed oil were indistinguishable from wild-type controls in growth parameters and seed oil content. By contrast, lines with more than 80% oleate in the seed oil had significantly lower seedling establishment and vigor, delayed flowering and reduced plant height at maturity. These lines also had 7-11% reductions in seed oil content. Our results extend understanding of the B. napusFAD2 isozymes and define the practical limit to increasing oil oleate content in this crop species.
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Details
- Title
- Identification, characterization and field testing of Brassica napus mutants producing high-oleic oils
- Creators
- Shuangyi Bai - Washington State UniversitySteven Engelen - BASF Agr Solut Belgium NV, Technol Pk 101, B-9052 Ghent, BelgiumPeter Denolf - BASF Agr Solut Belgium NV, Technol Pk 101, B-9052 Ghent, BelgiumJames G. Wallis - Washington State University, UNKNOWNKatherine Lynch - Washington State Univ, Inst Biol Chem, Clark Hall, Pullman, WA 99164 USAJesse D. Bengtssonl - Washington State Univ, Inst Biol Chem, Clark Hall, Pullman, WA 99164 USAMichel Van Thournout - BASF Agr Solut Belgium NV, Technol Pk 101, B-9052 Ghent, BelgiumBoris Haesendonckx - BASF Agr Solut Belgium NV, Technol Pk 101, B-9052 Ghent, BelgiumJohn Browse - Washington State University, Institute of Biological Chemistry
- Publication Details
- The Plant journal : for cell and molecular biology, Vol.98(1), pp.33-41
- Academic Unit
- Department of Pharmaceutical Sciences
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- 2018-67013-27459 / BASF Agricultural Solutions by Agriculture and Food Research Initiative (AFRI) competitive award from the USDA National Institute of Food and Agriculture Agricultural Research Center at Washington State University T32GM008336 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Identifiers
- 99901181236101842
- Language
- English
- Resource Type
- Accepted manuscript