Large deletions in the CBF gene cluster at the Fr-B2 locus are associated with reduced frost tolerance in wheat

Stephen Pearce; Jie Zhu; Ákos Boldizsár; Attila Vágújfalvi; Adrienne Burke; Kimberley Garland-Campbell; Gábor Galiba; Jorge Dubcovsky

doi:10.1007/s00122-013-2165-y

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Large deletions in the CBF gene cluster at the Fr-B2 locus are associated with reduced frost tolerance in wheat

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Large deletions in the CBF gene cluster at the Fr-B2 locus are associated with reduced frost tolerance in wheat

Stephen Pearce, Jie Zhu, Ákos Boldizsár, Attila Vágújfalvi, Adrienne Burke, Kimberley Garland-Campbell, Gábor Galiba and Jorge Dubcovsky

Theoretical and applied genetics, Vol.126(11), pp.2683-2697

11/2013

DOI: https://doi.org/10.1007/s00122-013-2165-y

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https://hdl.handle.net/2376/116374

PMCID: PMC4779059

PMID: 23884601

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Abstract

Triticum

cold acclimation

wheat

deletion

CBF

frost tolerance

Wheat plants which are exposed to periods of low temperatures (cold acclimation) exhibit increased survival rates when they are subsequently exposed to freezing temperatures. This process is associated with large-scale changes in the transcriptome which are modulated by a set of tandemly duplicated CBF ( C-repeat Binding Factor ) transcription factors located at the Fr-2 ( Frost Resistance-2 ) locus. While Arabidopsis has three tandemly duplicated CBF genes, the CBF family in wheat has undergone an expansion and at least 15 CBF genes have been identified, eleven of which are present at the Fr-2 loci on homoeologous group 5 chromosomes. We report here the discovery of three large deletions which eliminate six, nine, and all eleven CBF genes from the Fr-B2 locus in tetraploid and hexaploid wheat. In wild emmer wheat, the Fr-B2 deletions were found only among the accessions from the southern sub-populations. Among cultivated wheats, the Fr-B2 deletions were more common among varieties with a spring growth habit than among those with a winter growth habit. Replicated freezing tolerance experiments showed that both the deletion of nine CBF genes in tetraploid wheat and the complete Fr-B2 deletion in hexaploid wheat are associated with significant reductions in survival after exposure to freezing temperatures. Our results suggest that selection for the wild type Fr-B2 allele may be beneficial for breeders selecting for varieties with improved frost tolerance.

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Title: Large deletions in the CBF gene cluster at the Fr-B2 locus are associated with reduced frost tolerance in wheat
Creators: Stephen Pearce - Department of Plant Sciences, University of California, Davis, CA 95616. USA
Jie Zhu - USDA-ARS Wheat Genetics, Quality, Physiology and Disease Research Unit, Washington State University, Pullman WA 99164-6420, USA
Ákos Boldizsár - Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik u. 2, Martonvásár, H-2462, Hungary
Attila Vágújfalvi - Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik u. 2, Martonvásár, H-2462, Hungary
Adrienne Burke - USDA-ARS Wheat Genetics, Quality, Physiology and Disease Research Unit, Washington State University, Pullman WA 99164-6420, USA
Kimberley Garland-Campbell - USDA-ARS Wheat Genetics, Quality, Physiology and Disease Research Unit, Washington State University, Pullman WA 99164-6420, USA
Gábor Galiba - Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik u. 2, Martonvásár, H-2462, Hungary
Jorge Dubcovsky - Department of Plant Sciences, University of California, Davis, CA 95616. USA
Publication Details: Theoretical and applied genetics, Vol.126(11), pp.2683-2697
Academic Unit: Department of Crop and Soil Sciences
Identifiers: 99900547950501842
Language: English
Resource Type: Accepted manuscript