Pesticide and insect repellent mixture (permethrin and DEET) induces epigenetic transgenerational inheritance of disease and sperm epimutations

Mohan MANIKKAM; Rebecca TRACEY; Carlos GUERRERO-BOSAGNA; Michael K SKINNER

doi:10.1016/j.reprotox.2012.08.010

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Pesticide and insect repellent mixture (permethrin and DEET) induces epigenetic transgenerational inheritance of disease and sperm epimutations

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Pesticide and insect repellent mixture (permethrin and DEET) induces epigenetic transgenerational inheritance of disease and sperm epimutations

Mohan MANIKKAM, Rebecca TRACEY, Carlos GUERRERO-BOSAGNA and Michael K SKINNER

Reproductive toxicology (Elmsford, N.Y.), Vol.34(4), pp.708-719

2012

DOI: https://doi.org/10.1016/j.reprotox.2012.08.010

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https://hdl.handle.net/2376/103872

PMCID: PMC3513590

PMID: 22975477

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Abstract

Fundamental and applied biological sciences. Psychology

Embryology: invertebrates and vertebrates. Teratology

Pharmacology. Drug treatments

Biological and medical sciences

Antiparasitic agents

Pesticides, fertilizers and other agrochemicals toxicology

Medical sciences

Teratology. Teratogens

Antibiotics. Antiinfectious agents. Antiparasitic agents

Toxicology

Environmental compounds are known to promote epigenetic transgenerational inheritance of disease. The current study was designed to determine if a "pesticide mixture" (pesticide permethrin and insect repellent N,N-diethyl-meta-toluamide. DEET) promotes epigenetic transgenerational inheritance of disease and associated DNA methylation epimutations in sperm. Gestating F0 generation female rats were exposed during fetal gonadal sex determination and the incidence of disease evaluated in F1 and F3 generations. There were significant increases in the incidence of total diseases in animals from pesticide lineage F1 and F3 generation animals. Pubertal abnormalities, testis disease, and ovarian disease (primordial follicle loss and polycystic ovarian disease) were increased in F3 generation animals. Analysis of the pesticide lineage F3 generation sperm epigenome identified 363 differential DNA methylation regions (DMR) termed epimutations. Observations demonstrate that a pesticide mixture (permethrin and DEET) can promote epigenetic transgenerational inheritance of adult onset disease and potential sperm epigenetic biomarkers for ancestral environmental exposures. (C) 2012 Elsevier Inc. All rights reserved.

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Title: Pesticide and insect repellent mixture (permethrin and DEET) induces epigenetic transgenerational inheritance of disease and sperm epimutations
Creators: Mohan MANIKKAM - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, United States
Rebecca TRACEY - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, United States
Carlos GUERRERO-BOSAGNA - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, United States
Michael K SKINNER - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, United States
Publication Details: Reproductive toxicology (Elmsford, N.Y.), Vol.34(4), pp.708-719
Academic Unit: Biological Sciences, School of
Publisher: Elsevier; Amsterdam
Number of pages: 12
Grant note: DOD; United States Department of Defense NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
Identifiers: 99900546561401842
Language: English
Resource Type: Accepted manuscript