Accepted manuscript
Photoswitchable Nanoparticles Enable High-Resolution Cell Imaging: The PULSAR Microscopy
Journal of the American Chemical Society, Vol.130(46), pp.15279-15281
11/19/2008
Handle:
https://hdl.handle.net/2376/108322
PMCID: PMC2651006
PMID: 18939833
Abstract
Beyond-diffraction-limit optical imaging of cells will reveal biological mechanisms, cellular structures, and physiological processes in nanometer scale. Harnessing the photoswitching properties of spiropyran fluorophores, we achieved nano-resolution fluorescence imaging using photo-actuated unimolecular logical switching attained reconstruction (PULSAR) microscopy. The PULSAR microscope successfully resolved nanostructures and subcellular organelles when the photoswitchable nanoparticles containing spiropyran dyes were used as the fluorescent probes.
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Details
- Title
- Photoswitchable Nanoparticles Enable High-Resolution Cell Imaging: The PULSAR Microscopy
- Creators
- Dehong Hu - Pacific Northwest National Laboratory, Richland, WA 99352, USAZhiyuan Tian - The Department of Chemistry, Washington State University, Pullman, WA 99164, USAWuwei Wu - The Department of Chemistry, Washington State University, Pullman, WA 99164, USAWei Wan - The Department of Chemistry, Washington State University, Pullman, WA 99164, USAAlexander D. Q Li - The Department of Chemistry, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Journal of the American Chemical Society, Vol.130(46), pp.15279-15281
- Academic Unit
- Department of Chemistry
- Publisher
- Amer Chemical Soc
- Number of pages
- 4
- Grant note
- R01GM065306 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) CHE-0805547 / National Science Foundation; National Science Foundation (NSF) GM065306 / National Institute of General Medicine Sciences; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Identifiers
- 99900547208801842
- Language
- English
- Resource Type
- Accepted manuscript