Accepted manuscript
Phylogenetic and expression analysis of the basic helix-loop-helix transcription factor gene family: genomic approach to cellular differentiation
Differentiation (London), Vol.76(9), pp.1006-1042
2008
Handle:
https://hdl.handle.net/2376/109253
PMCID: PMC5728086
PMID: 18557763
Abstract
A phylogenetic analysis of seven different species (human, mouse, rat, worm, fly, yeast, and plant) utilizing all (541) basic helix-loop-helix (bHLH) genes identified, including expressed sequence tags (EST), was performed. A super-tree involving six clades and a structural categorization involving the entire coding sequence was established. A nomenclature was developed based on clade distribution to discuss the functional and ancestral relationships of all the genes. The position/location of specific genes on the phylogenetic tree in relation to known bHLH factors allows for predictions of the potential functions of uncharacterized bHLH factors, including EST's. A genomic analysis using microarrays for four different mouse cell types (i.e. Sertoli, Schwann, thymic, and muscle) was performed and considered all known bHLH family members on the microarray for comparison. Cell-specific groups of bHLH genes helped clarify those bHLH genes potentially involved in cell specific differentiation. This phylogenetic and genomic analysis of the bHLH gene family has revealed unique aspects of the evolution and functional relationships of the different genes in the bHLH gene family.
Metrics
8 Record Views
Details
- Title
- Phylogenetic and expression analysis of the basic helix-loop-helix transcription factor gene family: genomic approach to cellular differentiation
- Creators
- Jeffrey D Stevens - Center for Reproductive Biology, School of Molecular Biosciences, Pullman, WA 99164-4231, USA; Tel: +1 509 335 1024; Fax: +1 509 335 2176Eric H Roalson - School of Biological Sciences, Washington State University, Pullman, WA 99164-4231, USAMichael K Skinner - Center for Reproductive Biology, School of Molecular Biosciences, Pullman, WA 99164-4231, USA; Tel: +1 509 335 1024; Fax: +1 509 335 2176
- Publication Details
- Differentiation (London), Vol.76(9), pp.1006-1042
- Academic Unit
- Biological Sciences, School of
- Publisher
- Elsevier B.V
- Grant note
- 5R01 HD04381-04 / NIH grant to Michael K. Skinner
- Identifiers
- 99900547198501842
- Language
- English
- Resource Type
- Accepted manuscript