Progesterone regulation of primordial follicle assembly in bovine fetal ovaries

Eric E Nilsson; Michael K Skinner

doi:10.1016/j.mce.2009.09.004

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Progesterone regulation of primordial follicle assembly in bovine fetal ovaries

Accepted manuscript

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Progesterone regulation of primordial follicle assembly in bovine fetal ovaries

Eric E Nilsson and Michael K Skinner

Molecular and cellular endocrinology, Vol.313(1-2), pp.9-16

12/10/2009

DOI: https://doi.org/10.1016/j.mce.2009.09.004

Handle:

https://hdl.handle.net/2376/114787

PMCID: PMC2757495

PMID: 19747959

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Abstract

Estradiol - metabolism

Oocytes - metabolism

Gene Expression Profiling

Fetus - drug effects

Oocytes - cytology

Ovarian Follicle - drug effects

Microarray Analysis

Ovarian Follicle - physiology

Progesterone - metabolism

Ovarian Follicle - cytology

Fetus - anatomy & histology

Progesterone - pharmacology

Organ Culture Techniques

Fertility in mammals is dependant on females having an adequate primordial follicle pool to supply oocytes for fertilization. The formation of primordial follicles is called ovarian follicular assembly. In rats and mice progesterone and estradiol have been shown to inhibit follicle assembly with assembly occurring after birth when the pups are removed from the high-steroid maternal environment. In contrast, primordial follicle assembly in other species, such as cattle and humans, occurs during fetal development before birth. The objective of the current study is to determine if progesterone levels regulate primordial follicle assembly in fetal bovine ovaries. Ovaries and blood were collected from bovine fetuses. Interestingly, ovarian progesterone and estradiol concentrations were found to decrease with increasing fetal age and correlated to increased primordial follicle assembly. Microarray analysis of fetal ovary RNA suggests that progesterone membrane receptor and estrogen nuclear receptor are expressed. Treatment of fetal bovine ovary cultures with a higher progesterone concentration significantly decreased primordial follicle assembly. Observations indicate that progesterone affects ovarian primordial follicle assembly in cattle, as it does in rats and mice.

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Title: Progesterone regulation of primordial follicle assembly in bovine fetal ovaries
Creators: Eric E Nilsson - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99163-4231, United States
Michael K Skinner
Publication Details: Molecular and cellular endocrinology, Vol.313(1-2), pp.9-16
Academic Unit: Biological Sciences, School of
Publisher: Ireland
Grant note: R01 HD048898-04 / NICHD NIH HHS R01 ES012974 / NIEHS NIH HHS R01 HD048898 / NICHD NIH HHS
Identifiers: 99900547802801842
Language: English
Resource Type: Accepted manuscript