Single-chromophore-based photoswitchable nanoparticles enable dual-alternating-color fluorescence for unambiguous live cell imaging

Zhiyuan Tian; Wuwei Wu; Wei Wan; Alexander D Q Li

doi:10.1021/ja805150g

Back

Single-chromophore-based photoswitchable nanoparticles enable dual-alternating-color fluorescence for unambiguous live cell imaging

Accepted manuscript

Open access

Single-chromophore-based photoswitchable nanoparticles enable dual-alternating-color fluorescence for unambiguous live cell imaging

Zhiyuan Tian, Wuwei Wu, Wei Wan and Alexander D Q Li

Journal of the American Chemical Society, Vol.131(12), pp.4245-4252

04/01/2009

DOI: https://doi.org/10.1021/ja805150g

Handle:

https://hdl.handle.net/2376/104819

PMCID: PMC2667703

PMID: 19275146

Files and links (2)

pdf

nihms1018482.04 MBDownload View

Open Access

url

https://doi.org/10.1021/ja805150gView

Published (Version of record) Open

Abstract

Fluorescent Dyes - chemistry

Reproducibility of Results

Acrylates - chemistry

Models, Chemical

Nanoparticles - chemistry

Nanotechnology - methods

Humans

Acrylamide - chemistry

Endocytosis

Microscopy, Fluorescence - methods

Photochemistry - methods

Fluorescence Resonance Energy Transfer

Polymers - chemistry

Kinetics

HMGA Proteins - chemistry

We have developed a class of spiropyran dyes and their fluorescence colors can be reversibly photoswitched from red to green, blue, or nearly dark, thus alternating between two colors. Such individual dyes emit either one color or the other but not both simultaneously. Nanoparticles enabled with these photoswitchable dyes, however, emit either one pure color or a combination of both colors because the nanoparticle fluorescence originates from multiple dyes therein. As a result, the nanoparticle shines >30 times brighter than state-of-the-art organic dyes such as fluorescein. Interestingly, these copolymer nanoparticles exhibit tunable nonspecific interactions with live cells, and nanoparticles containing properly balanced butyl acrylate and acrylamide monomers render essentially very little nonspecific binding to live cells. Decorated with HMGA1 protein, these optically switchable dual-color nanoparticles undergo endocytosis and unambiguously identify themselves from fluorescence interference including autofluorescence, thus enabling a new tool for live cell imaging.

Metrics

20 Record Views

Details

Title: Single-chromophore-based photoswitchable nanoparticles enable dual-alternating-color fluorescence for unambiguous live cell imaging
Creators: Zhiyuan Tian - Department of Chemistry and Center for Materials Research, Washington State University, Pullman, Washington 99164, USA
Wuwei Wu
Wei Wan
Alexander D Q Li
Publication Details: Journal of the American Chemical Society, Vol.131(12), pp.4245-4252
Academic Unit: Department of Chemistry
Publisher: United States
Number of pages: 8
Grant note: R01 GM065306-04 / NIGMS NIH HHS GM065306 / NIGMS NIH HHS R01 GM065306-05 / NIGMS NIH HHS R01 GM065306 / NIGMS NIH HHS
Identifiers: 99900546855601842
Language: English
Resource Type: Accepted manuscript