Book chapter
Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G-Proteins in Arabidopsis
G Protein-Coupled Receptor Signaling in Plants, pp.45-54
Methods in Molecular Biology, Humana Press
07/17/2013
Handle:
https://hdl.handle.net/2376/105376
PMID: 23913034
Abstract
Heterotrimeric GTP-binding proteins (G-proteins) and G-protein-coupled receptors are important signaling components in eukaryotes. In plants, the G-proteins are involved in diverse physiological processes, some of which are exerted via changes in the level of cytosolic free calcium concentration ([Ca2+]cyt). Various techniques have been developed to measure the change of [Ca2+]cyt, e.g., calcium-sensitive microelectrodes, chemical fluorescent dyes, and biosensors based on luminescent or fluorescent indicators. In this chapter, we describe a protocol for in vivo [Ca2+]cyt measurement in G-protein mutants expressing aequorin, a luminescent-based calcium biosensor, to extend our knowledge about G-protein mediated Ca2+ signaling. This method is also applicable to other early signaling events that are mediated by changes in [Ca2+]cyt levels.
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Details
- Title
- Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G-Proteins in Arabidopsis
- Creators
- Kiwamu Tanaka - Divisions of Plant Science and Biochemistry, University of Missouri, Columbia, USAJeongmin Choi - Divisions of Plant Science and Biochemistry, University of Missouri, Columbia, USAGary Stacey - Divisions of Plant Science and Biochemistry, University of Missouri, Columbia, USA
- Publication Details
- G Protein-Coupled Receptor Signaling in Plants, pp.45-54
- Academic Unit
- Plant Pathology, Department of
- Series
- Methods in Molecular Biology
- Publisher
- Humana Press; Totowa, NJ
- Identifiers
- 99900546670601842
- Language
- English
- Resource Type
- Book chapter