Conference proceeding
Production of Cellulase/β-Glucosidase by the Mixed Fungi Culture of Trichoderma reesei and Aspergillus phoenicis on Dairy Manure
Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals, pp.93-104
ABAB Symposium
Symposium on Biotechnology for Fuels and Chemicals, 26
2005
Handle:
https://hdl.handle.net/2376/111033
Abstract
A cellulase production process was developed by growing the fungi Trichoderma reesei and Aspergillus phoenicis on dairy manure. T. reesei produced a high total cellulase titer (1.7 filter paper units [FPU]/mL, filter paper activity) in medium containing 10 g/L of manure (dry basis [w/w]), 2 g/L KH2PO4, 2 mL/L of Tween-80, and 2mg/L of CoCl2. However, β-glucosidase activity in the T. reesei-enzyme system was very low. T. reesei was then cocultured with A. phoenicis to enhance the β-glucosidase level. The mixed culture resulted in a relatively high level of total cellulase (1.54 FPU/mL) and β-glucosidase (0.64 IU/mL). The ratio of β-glucosidase activity to filter paper activity was 0.41, suitable for hydrolyzing manure cellulose. The crude enzyme broth from the mixed culture was used for hydrolyzing the manure cellulose, and the produced glucose was significantly (p < 0.01) higher than levels obtained by using the commercial enzyme or the enzyme broth of the pure culture T. reesei.
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Details
- Title
- Production of Cellulase/β-Glucosidase by the Mixed Fungi Culture of Trichoderma reesei and Aspergillus phoenicis on Dairy Manure
- Creators
- Zhiyou Wen - Department of Biological Systems Engineering, Washington State University, PullmanWei Liao - Department of Biological Systems Engineering, Washington State University, PullmanShulin Chen - Department of Biological Systems Engineering, Washington State University, Pullman
- Publication Details
- Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals, pp.93-104
- Conference
- Symposium on Biotechnology for Fuels and Chemicals, 26
- Academic Unit
- Biological Systems Engineering, Department of
- Series
- ABAB Symposium
- Publisher
- Humana Press; Totowa, NJ
- Identifiers
- 99900582332901842
- Language
- English
- Resource Type
- Conference proceeding