Dissertation
3D PRINTED CALCIUM PHOSPHATE SCAFFOLDS AND COATINGS ON TITANIUM ALLOYS FOR OTHOPEDIC APPLICATIONS: EFFECTS OF DOPANTS AND NATURAL MEDICINAL COMPOUNDS ON BIOLOGICAL PROPERTIES
Doctor of Philosophy (PhD), Washington State University
01/2019
Handle:
https://hdl.handle.net/2376/118004
Abstract
This study intends to understand the effects of dopants and natural medicinal compounds with osteogenic potential to improve bone formation leading to accelerated healing and improved implant performance.
Doped hydroxyapatite coatings were developed on Ti alloys and were dip coated by acemannan to analyze its effects on the neo bone formation and chitosan to analyze the effects of polymer coating on the release kinetics. Chitosan leads to a controlled release of acemannan for 30 days. In vitro results demonstrate an enhanced osteoblast cell viability and proliferation by acemannan after 2 and 5 days of culture. Acemannan improved neo bone formation at early points in a rat distal femur defect model and chitosan enhanced mineralization. The bioavailability of acemannan was found to peak at ~25-30 days in a rat body.
Increased systematic pH concentrations locally by the release of sodium bicarbonate diminished acidosis and alleviated malignancy. The same of dosage of doxorubicin inhibited both osteoblast and osteosarcoma cell attachment and viability whereas, sodium bicarbonate abated osteosarcoma cell proliferation. Sodium bicarbonate also inhibited osteoblast cell proliferation in the early time points, however, the cell viability increased after the initial burst release of the molecule. A coating of polycaprolactone (PCL) controlled the initial burst release of bicarbonate, which enhanced the osteoblast cell viability, but was capable of diminishing osteosarcoma cell proliferation.
Calcium phosphate scaffolds with 3D-interconnected channels were fabricated to provide pathways for micronutrients and improved cell-material interactions through bioactive fixation. Gingerol, an antioxidant and anti-inflammatory molecule, enhances osteoblastic activity in addition to its anti-osteoclastic activity. However, due to its poor solubility and high intestinal liver metabolism, it showed limited oral efficacy in various preclinical and clinical studies. To enhance its bioavailability and to provide higher release, PCL and polyethylene glycol (PEG) were used as the polymeric system to enable continuous release of gingerol from the hydroxyapatite matrix for 25 days. Enhanced cell viability was observed with gingerol-PCL-PEG in static and dynamic culture conditions.
Results show that local release of natural medicines and dopants has the potential for being used for wound healing and tissue regeneration applications in bone tissue engineering.
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Details
- Title
- 3D PRINTED CALCIUM PHOSPHATE SCAFFOLDS AND COATINGS ON TITANIUM ALLOYS FOR OTHOPEDIC APPLICATIONS: EFFECTS OF DOPANTS AND NATURAL MEDICINAL COMPOUNDS ON BIOLOGICAL PROPERTIES
- Creators
- Dishary Banerjee
- Contributors
- Susmita Bose (Advisor)Amit Bandyopadhyay (Committee Member)William Dernell (Committee Member)Yuehe Lin (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- School of Mechanical and Materials Engineering
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 295
- Identifiers
- 99900581504601842
- Language
- English
- Resource Type
- Dissertation