Dissertation
ADVANCED MICROSCOPY IMAGING OF PHLOEM PROTEINS IN ARABIDOPSIS REVEAL INSIGHTS INTO MUNCH'S PRESSURE FLOW HYPOTHESIS
Doctor of Philosophy (PhD), Washington State University
01/2014
Handle:
https://hdl.handle.net/2376/5119
Abstract
Phloem proteins have been widely regarded as a wound response mechanism. All imagery showing apparent occlusions of this protein at a sieve plate have been dismissed as preparation artifact and ignored. Unfortunately, these images only show one still frame of a movie and so all conclusions are susceptible to misinterpretation. Presented here is a combination of high resolution still images with complete context from a dynamic in vivo reference. This new perspective shows that not only are the Sieve Element Occluding Related (SEOR) phloem protein agglomerations in Arabidopsis common in healthy, translocating, uninjured plants, but that they do not appear to occlude the phloem at all. The previously known purpose of this very common family of proteins is once again obscure.
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Details
- Title
- ADVANCED MICROSCOPY IMAGING OF PHLOEM PROTEINS IN ARABIDOPSIS REVEAL INSIGHTS INTO MUNCH'S PRESSURE FLOW HYPOTHESIS
- Creators
- Daniel Robert Froelich
- Contributors
- Michael Knoblauch (Advisor)Hanjo Hellmann (Committee Member)Ray Lee (Committee Member)Winifried Peters (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Biological Sciences, School of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 202
- Identifiers
- 99900581844301842
- Language
- English
- Resource Type
- Dissertation