BENEFICIAL EFFECTS OF PURPLE POTATO ON GASTROINTESTINAL HEALTH

Qi Sun

doi:10.7273/000006501

The gastrointestinal tract (GIT) is vital for nutrient digestion, absorption, and host defense. Several diseases, such as inflammatory bowel disease (IBD) and colorectal cancer, can compromise the GIT barrier, impacting epithelial cell metabolism, function, and gut microflora. This dissertation evaluated the potential benefits of purple-fleshed potatoes (PP), known for their rich macronutrient and phytochemical content, and their polyphenol-rich extract supplementation on intestinal epithelial barrier function, metabolism, and the microbial metabolome using a murine colitis model induced by dextran sulfate sodium (DSS) and intestinal epithelial cell line. The study also examined the influence of different cooking methods on the antioxidant capacity and anti-inflammatory capacity of PP bioactive. Results indicated PP, regardless of cooking methods, retained its phytochemical content, antioxidant, and anti-inflammatory activities. Among the tested cooking methods, vacuum-sealing and boiling exhibit the highest content of extractable polyphenolic compounds and bioactivity. PP extract promoted differentiation, up-regulated the level of peroxisome proliferator-activated receptor γ coactivator (PGC)-1α and enhanced mitochondrial biogenesis in human intestinal epithelial Caco-2 cells. Inhibition of PGC-1α by either inhibitor or siRNA knockdown negated the enhancing effects of PP extract on the expression of differentiation and mitochondrial biogenesis markers. Furthermore, purple potato (PP) extract inhibited HIF-1α accumulation and the expression of its downstream genes in Caco-2 cells. PP extract reduced cellular lactate production induced by hypoxia. PP extract suppressed hypoxia-induced shifts in cellular glycolytic metabolism. The fecal metabolome analyses in DSS-induced colitis mice revealed significant alterations in 68 compounds, primarily encompassing amino acids, fatty acids, organic acids, carbohydrates, and polyamines. DSS treatment led to the upregulation of 25 metabolites mainly amino acids and fatty acids in feces. However, dietary PP supplementation reversed 13 of the 25 metabolites affected by DSS. Additionally, six potential microbial metabolites derived from PP polyphenols were identified, showing a strong positive correlation with M2 differentiation of macrophages. In summary, these findings highlight the beneficial effects of PP on intestinal epithelium differentiation, cellular metabolism, and modulation colitis-induced alteration in intestinal microbial metabolome.

BENEFICIAL EFFECTS OF PURPLE POTATO ON GASTROINTESTINAL HEALTH

Files and links (1)

Abstract

Metrics

Details