Dissertation
CHARACTERIZATION OF FLAVANOID ENZYMES IN GRASSES
Washington State University
Doctor of Philosophy (PhD), Washington State University
05/2024
DOI:
https://doi.org/10.7273/000006468
Abstract
Sorghum and switchgrass are widely cultivated cereal crops that have been identified as a focus for both feedstock and bioenergy due to their resilience to large temperature changes, water need, and high concentrations of secondary metabolites. One of these secondary metabolites is a group of phytochemicals called flavonoids, characterized by their C(6)-C(3)-C(6) structure. Flavonoids are adjacent to the monolignol pathway and divert biomass from lignin via the committed enzyme Chalcone Synthase. The enzymes of the flavonoid pathway are hypothesized to form a metabolon around a membrane bound P450 enzyme dependent on metabolic needs and species. A detailed understanding of the structural, enzymatic, and metabolon formation of flavonoids in grasses could enable structural tailoring of the pathway to benefit feedstock and biofuel. High-resolution crystallographic structures, enzymatic analysis, and protein-protein interactions are presented in this thesis to aid in the elucidation of fundamental knowledge to be utilized for future engineering.
Metrics
2 File views/ downloads
23 Record Views
Details
- Title
- CHARACTERIZATION OF FLAVANOID ENZYMES IN GRASSES
- Creators
- Jacob Alexander Lewis
- Contributors
- ChulHee Kang (Chair)James Brozik (Committee Member)Clifford Berkman (Committee Member)Erika Offerdahl (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Chemistry
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 175
- Identifiers
- 99901121439501842
- Language
- English
- Resource Type
- Dissertation