Dissertation
CHARACTERIZATION OF THE TRANSCRIPTOME AND PROTEOME OF A SALMONELLA ENTERICA SEROVAR TYPHIMURIUM DNA ADENINE METHYLTRANSFERASE MUTANT
Doctor of Philosophy (PhD), Washington State University
01/2014
Handle:
https://hdl.handle.net/2376/5209
Abstract
Non-typhoidal Salmonella serotypes, including Salmonella enterica serovar Typhimurium, are food-borne pathogens and important causes of bacterial enteric disease in both humans and livestock. Infection of livestock is a common source of transmission of Salmonella into the human food supply, and therefore interventions decreasing infection in livestock species have the potential to reduce the burden of human infection. Vaccination with Salmonella enterica serovar Typhimurium lacking DNA adenine methyltransferase (Dam) confers cross-protective immunity against multiple Salmonella serotypes. The mechanistic basis is thought to be associated with the de-repression of genes that are tightly regulated when transiting from one microenvironment to another. De-repression provides a potential means for the production of a more highly expressed and stable antigenic repertoire capable of inducing cross-protective immune responses.
In our first study, we identified stably expressed and highly transcribed genes in the UK-1 dam mutant in comparison to the parental strain when transitioning from an extracellular to an intracellular environmental condition. These findings were supported with comparison of two additional, clinically relevant Salmonella serotypes, Dublin and Newport, allowing us to identify a core gene repertoire whose transcription may play a role in inducing cross-protective immune response. In our second study, we characterized protein expression differences between the UK-1 dam mutant and wt in the same culture conditions. Significantly more proteins were expressed by the UK-1 dam mutant in comparison to wt on the surface of the bacteria and released into the culture supernatant, and these proteins represented a broad range of functional categories. Comparison of transcriptional and proteome differences between strains revealed poor correspondence however further analysis may reveal more subtle but important trends. In summary, many genes and proteins candidates identified in the UK-1 dam mutant could play a role in immune activity and development of protection afforded in vivo leading to many avenues for further research.
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Details
- Title
- CHARACTERIZATION OF THE TRANSCRIPTOME AND PROTEOME OF A SALMONELLA ENTERICA SEROVAR TYPHIMURIUM DNA ADENINE METHYLTRANSFERASE MUTANT
- Creators
- Claire Burbick Miller
- Contributors
- Kevin K Lahmers (Advisor)Wendy C Brown (Committee Member)Devendra H Shah (Committee Member)Robert H Mealey (Committee Member)Douglas R Call (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 72
- Identifiers
- 99900581533201842
- Language
- English
- Resource Type
- Dissertation