Dissertation
CLONING OF THE SEEDLING SPOT BLOTCH RESISTANCE GENE Rcs5
Doctor of Philosophy (PhD), Washington State University
01/2011
Handle:
https://hdl.handle.net/2376/117902
Abstract
The focus of my thesis was to investigate and clone the seedling spot blotch resistance gene, Rcs5. Using a positional cloning approach and molecular markers derived by using synteny with related sequenced genomes of rice and Brachypodium, I was able to saturate the Rcs5 region, identify a small interval that is likely to contain Rcs5, and sequence six BACs from the resistant cultivar Morex that should contain Rcs5. Allele sequencing and recombinant analysis limited the number of candidate genes to two Wall-associated kinases, ctg19 and ctg37. These genes represent a novel class of resistance genes in which only WAK1 and WAKL22 from Arabidopsis have been shown to be involved in disease resistance. This research will add to the understanding of disease resistance to hemibiotrophic organisms in crop species.
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Details
- Title
- CLONING OF THE SEEDLING SPOT BLOTCH RESISTANCE GENE Rcs5
- Creators
- Tom B Drader
- Contributors
- Andris Kleinhofs (Advisor)Diter Von Wettstein (Committee Member)Patricia Okubara (Committee Member)Gary Thorgaard (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Molecular Biosciences, School of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 124
- Identifiers
- 99900581661501842
- Language
- English
- Resource Type
- Dissertation