Dissertation
Characterization of amino acid transporter function in Arabidopsis thaliana
Washington State University
Doctor of Philosophy (PhD), Washington State University
12/2008
DOI:
https://doi.org/10.7273/000005846
Abstract
Amino acids are the main transport form of organic nitrogen in plants and their import into plant cells is predicted to be by plasma membrane transport proteins. This research analyzed the roles of the amino permease AtAAP1 as well as AtLHT amino acid transporters in Arabidopsis with an emphasis on amino acid uptake by the roots and transport within flowers. Expression of AtAAP1 and AtLHT6 was localized to root cells, including root hairs. Furthermore, the transporters were targeted to the plasma membrane, indicating a function in nitrogen import into cells. Transport studies were performed with aap1 and lht6 seedlings and showed reduced amino acid uptake in mutants compared to wild type. Moreover, aap1 mutants could grow on media containing amino acid concentrations that are toxic to wild type, and plants overexpressing AAP1 displayed increased uptake of amino acids. Together, these data demonstrate that transporters are important for root amino acid acquisition and that genetic manipulation of root located amino acid import systems results in alteration of nitrogen uptake.
Metrics
5 File views/ downloads
22 Record Views
Details
- Title
- Characterization of amino acid transporter function in Arabidopsis thaliana
- Creators
- Justin Joe Foster
- Contributors
- Mechthild Tegeder (Advisor) - Washington State University, School of Biological SciencesGerry Edwards (Committee Member)Vincent R Franceschi (Committee Member)Andrew McCubbin (Committee Member) - Washington State University, School of Biological Sciences
- Awarding Institution
- Washington State University
- Academic Unit
- School of Biological Sciences
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 190
- Identifiers
- 99901055039101842
- Language
- English
- Resource Type
- Dissertation