Files and links (1)
Arjak FINAL thesis_MAIN_Dec_2110.43 MB
Embargoed Access, Embargo ends: 07/01/2027
Dissertation
Curcumin, Gingerol, and Natural Polymer Loaded 3D Printed Calcium Phosphate Scaffolds and Coated Ti Implants for Orthopedic and Dental Applications
Washington State University
Doctor of Philosophy (PhD), Washington State University
2022
DOI:
https://doi.org/10.7273/000005042
Abstract
A significant number of the world’s population is affected by several musculoskeletal disorders including bone cancer, birth defects, war injuries, and osteoporosis. The increase in the average median age of the population and overactive lifestyle of younger people is expected to enhance the rate of these disorders in younger patients as well. This alarming scenario regarding musculoskeletal disorders is continuously fueling the research on bio implants and scaffolds with longer service life. The objective of this research is to understand the effects of naturally derived biomolecules such as curcumin from turmeric, gingerol from ginger, essential elements, and natural polymers on the osteogenic, angiogenic, antibacterial, and in vitro chemo-preventive properties after direct incorporation on 3D printed defect specific scaffolds and plasma sprayed coatings. Innovative optimization methods utilizing computational multi-objective optimization strategies lead to a significant enhancement of the mechanical and biological properties of the 3D printed polymer-ceramic composite scaffolds, constructed with Zn2+ modified starch and hydroxyapatite (HA).
Chemical modification of curcumin using polymer-metal-drug complexation leads to ~ 2.5 times enhancement in drug release in biological media at pH 5.0 and 7.4, as compared to only curcumin. The presence of curcumin shows up to ~ 9 times lesser osteosarcoma viability than that of the control after 11 days of cell culture. The antibacterial efficacy results against both gram-positive and gram-negative bacteria indicate that the designed scaffolds with curcumin or gingerol show up to ~ 90 % antibacterial efficacy and rupture in the bacterial cell wall. The dual effects of these two naturally derived biomolecules after direct incorporation on the plasma-coated implants show significantly enhanced (~ 2.5 times) in vivo bone formation in rat distal femur model after 6-weeks of implantation. The new bone formation is quantified with different staining methods such as Hematoxylin and eosin (H&E) staining, Sanderson’s rapid bone staining (SRBS), and Movat Penta Chrome staining. The new blood vessel formation in presence of dopants such as MgO is quantified using von Willebrand Factor (vWF) staining. These scaffolds and coatings with enhanced biological properties can be utilized for low load -bearing and load-bearing defect repair sites respectively.
Metrics
37 Record Views
Details
- Title
- Curcumin, Gingerol, and Natural Polymer Loaded 3D Printed Calcium Phosphate Scaffolds and Coated Ti Implants for Orthopedic and Dental Applications
- Creators
- Arjak Bhattacharjee
- Contributors
- Susmita Bose (Advisor)Amit Bandyopadhyay (Committee Member) - Washington State University, School of Mechanical and Materials EngineeringWilliam S. Dernell (Committee Member)David P. Field (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- School of Mechanical and Materials Engineering
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 356
- Identifiers
- 99901019537501842
- Language
- English
- Resource Type
- Dissertation