Dissertation
Development of the bovine Ileal cannulation model to study Johne's disease and the role of Mycobacterium avium subsp. paratuberculosis in the pathogenesis of Crohn's disease
Washington State University
Doctor of Philosophy (PhD), Washington State University
12/2009
DOI:
https://doi.org/10.7273/000006210
Abstract
The purpose of this study was to test two hypotheses: Hypothesis 1. Human origin isolates of Map cause the same immune responses and pathological changes as bovine origin isolates of Map; Hypothesis 2. CD4 Lymphocyte depletion in the Ileal cannulation model will enhance establishment of paratuberculosis and accelerate pathogenesis. In the first experiment we developed a bovine ileal cannulation model, established a wild-type Map infection in the model, and characterized the infectious process and the local and systemic immune response. We successfully surgically cannulated calves and established infections, with significant systemic immune responses. In the second experiment we compared the infectivity, local, and systemic immune responses of calves experimentally infected with either bovine-origin Map or human-origin Map isolates using the ileal cannulation model. We demonstrated that the isolates of Map cultured from Crohn s disease patients were able to infect and cause a similar immune response to bovine isolates of Map. In the final experiment we transiently depleted CD4 T Lymphocytes in the early stages of infection in the ileal cannulation model and monitored infection and characterized the immune responses. CD4 depletion in the early stages of infection did not enhance establishment of paratuberculosis or accelerate pathogenesis in the ileal cannulation model.
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Details
- Title
- Development of the bovine Ileal cannulation model to study Johne's disease and the role of Mycobacterium avium subsp. paratuberculosis in the pathogenesis of Crohn's disease
- Creators
- Andrew J. Allen
- Contributors
- George M. Barrington (Chair)William C Davis (Committee Member) - Washington State University, Department of Veterinary Microbiology and PathologyThomas Eugene Besser (Committee Member) - Washington State University, Department of Veterinary Microbiology and PathologyJOHN MILES GAY (Committee Member)KEVIN KARL LAHMERS (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- College of Veterinary Medicine
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 104
- Identifiers
- 99901055127101842
- Language
- English
- Resource Type
- Dissertation