Dissertation
ELECTROCHEMICAL SCAFFOLD FOR WOUND-RELATED BIOFILM CONTROL
Doctor of Philosophy (PhD), Washington State University
01/2016
Handle:
https://hdl.handle.net/2376/108344
Abstract
This research aims to develop a novel electrochemical scaffold (e-scaffold) capable of continuous controlled delivery of sufficient concentration of H2O2 at a constant potential that can destroy wound-related biofilms without damaging host tissue. E-scaffolds were made of conductive carbon fabric and the operating potential was standardized to electrochemically reduce oxygen to generate desired concentration of H2O2. E-scaffold generated H2O2 effectively destroyed Acinetobacter baumannii biofilms in vitro and from infected dermal explants with no observable damage in underlying tissue. Furthermore, e-scaffold in combination with maltodextrin was applied against Gram-negative Acinetobacter baumannii and Gram-positive Staphylococcus aureus biofilm cells to improve efficacy in biofilm treatment. The combination of e-scaffold and maltodextrin treatment showed an inverted “U-shaped” or biphasic maltodextrin dose-dependent response in decreasing cell viability. Effectiveness of e-scaffold against persister cells and when combined with antibiotic, its ability to improve antibiotic efficacy against e-scaffold treated biofilms were also established. Mechanistic studies revealed that e-scaffold induced intracellular hydroxyl free radicals (OH•) and increased membrane permeability, which enhanced antibiotic susceptibility in biofilms. Overall, the scope of e-scaffold as an alternative wound-related biofilm treatment strategy was established. Finally, the goal and major findings of this research is summarized as concluding remarks. Some future works using e-scaffolds for electrochemical generation of other biocides and combined application of e-scaffold with other antimicrobial against multispecies mixed biofilm have been suggested.
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Details
- Title
- ELECTROCHEMICAL SCAFFOLD FOR WOUND-RELATED BIOFILM CONTROL
- Creators
- Sujala Tajneen Sultana
- Contributors
- Haluk Beyenal (Advisor)Alla Kostyukova (Committee Member)Douglas R Call (Committee Member)Haluk Resat (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 220
- Identifiers
- 99900581635801842
- Language
- English
- Resource Type
- Dissertation