Dissertation
ENGINEERING YARROWIA LIPOLYTICA AS A SAFE YEAST CELL BIOREFINERY PLATFORM FOR THE SUSTAINABLE PRODUCTION OF PHARMACEUTICALS AND BIOFUEL
Doctor of Philosophy (PhD), Washington State University
01/2017
Handle:
https://hdl.handle.net/2376/112053
Abstract
Today’s biotechnologists seek new cell factories to meet the growing demand for pharmaceuticals and fuels. This dissertation study evaluates the potential use of Y. lipolytica as a safe oleaginous cell factory platform for the sustainable production of fatty acid based-biofuels and bioproducts. This highlights challenges and strategies in the development of this platform along with the future applications. Y. lipolytica was designed and engineered at various metabolic levels of lipid biosynthesis, degradation, and regulation for the production of lipid, citric acid, ω-hydroxy fatty acid-16, and long chain dicarboxylic acids (LCDCA-16 & 18). Several genetic engineering strategies were used to achieve constitutive lipid accumulation and citric acid secretion in shake flask and bioreactor fermentation. A unique biosynthetic route was proposed for the de novo production of long chain dicarboxylic acid monomers from unrelated hydrophilic (non-oily) feedstock. These findings lay the foundation for the development of Y. lipolytica as a safe biorefinery platform for the sustainable production of high-value LCDCAs, with pharmaceutical and food uses, from renewable resources, including sugars and glycerol.
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Details
- Title
- ENGINEERING YARROWIA LIPOLYTICA AS A SAFE YEAST CELL BIOREFINERY PLATFORM FOR THE SUSTAINABLE PRODUCTION OF PHARMACEUTICALS AND BIOFUEL
- Creators
- Ali Abghari
- Contributors
- Shulin Chen (Advisor)Birgitte Ahring (Committee Member)Manuel Garcia-Pérez (Committee Member)Scott E. Baker (Committee Member)Xiaochao Xiong (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Biological Systems Engineering
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 186
- Identifiers
- 99900581514301842
- Language
- English
- Resource Type
- Dissertation