Dissertation
ESTROGEN AND PROGESTERONE REGULATION OF THE OVIDUCTAL ENVIRONMENT DURING PREIMPLANTATION EMBRYO DEVELOPMENT AND EMBRYO TRANSPORT
Washington State University
Doctor of Philosophy (PhD), Washington State University
01/2022
DOI:
https://doi.org/10.7273/000004644
Handle:
https://hdl.handle.net/2376/124788
Abstract
Ten to twenty percent of pregnancies end in miscarriage with eighty percent of pregnancy loss occurring during the first trimester. Because we lack a complete understanding of the environment within the oviduct (Fallopian tube in humans) and its role in regulating early pregnancy, it is difficult to provide diagnostic tools for oviductal origin of early pregnancy loss. It is well established that estrogen (E2) and progesterone (P4) cause biochemical and histological changes in the uterus. However, it is not completely understood how E2 and P4 regulate the oviductal environment in vivo. In this study, we assessed the effect of E2 and P4 on each oviductal cell type, using an ovariectomized-hormone-replacement mouse model and single-cell RNA-sequencing (scRNA-seq). We found that each cell type in the oviduct responded to E2 and P4 distinctively, especially ciliated and secretory epithelial cells. Additionally, we found that epithelial/muscle progesterone receptor (PGR) expression and E2 target gene, insulin-like growth factor 1 (Igf1), are required in the oviduct for normal female fertility and regulates preimplantation embryo development and transport to the uterus. These findings shed light on the roles of E2 and P4 signaling in the oviduct and offer potential applications for women’s reproductive health, infertility, contraception and diagnostic tools of early pregnancy loss.
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Details
- Title
- ESTROGEN AND PROGESTERONE REGULATION OF THE OVIDUCTAL ENVIRONMENT DURING PREIMPLANTATION EMBRYO DEVELOPMENT AND EMBRYO TRANSPORT
- Creators
- Emily Alexandria McGlade
- Contributors
- Wipawee Winuthayanon (Advisor)Patricia Hunt (Committee Member)Jon Oatley (Committee Member)James Pru (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Molecular Biosciences, School of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 268
- Identifiers
- OCLC#: 1365769385; 99900898933801842
- Language
- English
- Resource Type
- Dissertation