Dissertation
EXPLORING FLAVIN BIOSYNTHESIS IN PLANTS AND BACTERIA
Washington State University
Doctor of Philosophy (PhD), Washington State University
01/2021
DOI:
https://doi.org/10.7273/000003117
Handle:
https://hdl.handle.net/2376/123845
Abstract
Flavins, a class of small molecules used as redox cofactors, are essential for all life forms. This dissertation reports the results of three studies into the diverse roles of flavins in multiple organisms. Following an introduction to the topic of flavins (chapter one), chapter two details an investigation of the kinetic properties of a novel GTP cyclohydrolase from Sinorhizobium meliloti, which functions early in the flavin biosynthesis pathway. Understanding the kinetics of this enzyme has implications for understanding the two flavin pathways in this and similar microorganisms. The third and fourth chapters of this dissertation describe the results of two studies in the model organism Arabidopsis, using a transgenic approach to gain information about the role of flavins in plants. One of these studies contains the results of a transcriptome analysis which attempts to elucidate regulatory mechanisms of the pathway and finds a potential tie between flavins and iron metabolism. The final chapter describes an observed link between altered flavin metabolism and flowering time, and experiments associated with characterizing this relationship and potential mechanisms. The results contained in this dissertation add to the wide body of literature supporting the conclusion that flavins are important and have diverse roles and effects in metabolism of multiple organisms.
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Details
- Title
- EXPLORING FLAVIN BIOSYNTHESIS IN PLANTS AND BACTERIA
- Creators
- Skylar Ariel Johnson
- Contributors
- Sanja Roje (Advisor)Karen Sanguinet (Committee Member)Asaph Cousins (Committee Member)Helmut Kirchhoff (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Program in Molecular Plant Sciences
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 111
- Identifiers
- 99900651794601842
- Language
- English
- Resource Type
- Dissertation