Dissertation
Enteric Methane Mitigation Strategies with Homoacetogens from Kangaroo
Washington State University
Doctor of Philosophy (PhD), Washington State University
2023
DOI:
https://doi.org/10.7273/000006297
Abstract
We examined the microbial population from kangaroo fecal samples that are capable of utilizing hydrogen and carbon dioxide to produce acetic acid, a metabolic process known as homoacetogenesis. Our Next Generation Sequence results showed a homoacetogenic culture comprising mainly of Clostridiales. This study investigates the implementation of this culture in conjunction with methanogenic chemical inhibitor 2-bromoethanesulfonate and almond shell biochar on an in-vitro rumen culture. Through a combination of strategies applied for methane mitigation and rumen bioaugmentation, a 2.5-fold increase in acetic acid was observed in the in vitro rumen cultures with no methane production detected. AS addition also showed a 28% increase in acetic acid production in the homoacetogenic consortia. Our studies demonstrate that homoacetogens from kangaroos can serve as an effective bioaugmentation tool to improve acetic acid production of the rumen system. Such advancements can potentially contribute to the optimization of rumen fermentation processes and may have practical implications for improved livestock feed efficiency and on lowering enteric methane emissions. Almond shell biochar was showed to be a promising tool to be used in combination with homoacetogens to enhance acetic acid yields. Nitrogen-doping almond shell biochar improved its affinity to the homoacetogens and promoted greater biofilm formation. Notably, our study proposes that the homoacetogens identified in kangaroos offer a promising microbial tool for establishing an alternative hydrogen sink to the rumen to lower methane emission while maintaining the robustness of the rumen and increasing its productivity.
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Details
- Title
- Enteric Methane Mitigation Strategies with Homoacetogens from Kangaroo
- Creators
- Renan Stefanini Lopes
- Contributors
- Birgitte K Ahring (Advisor)Manuel Garcia-Perez (Committee Member)Kristen Johnson (Committee Member)Stephen J Callister (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- College of Agricultural, Human, and Natural Resource Sciences
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 121
- Identifiers
- 99901086433601842
- Language
- English
- Resource Type
- Dissertation