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Dissertation
Fractionation and Characterization of a Pine Pyrolysis Bio-Oil and In Vivo and In Silico Analysis of Its Anti-IL-17 Effects on HaCaT Human Keratinocytes
Washington State University
Doctor of Philosophy (PhD), Washington State University
2023
DOI:
https://doi.org/10.7273/000006321
Abstract
Psoriasis is an incurable skin disease affecting about 2-3% of the world's population. Interleukin 17 (IL-17) is a proinflammatory cytokine recognized to play a crucial role in psoriasis etiology and evolution. Monoclonal antibodies targeting IL-17A (secukinumab, ixekizumab) or its receptor (brodalumab) are available for treating psoriasis. However, their high costs, impossibility of use by topical route, and associated adverse events generate a need for identifying new anti-IL17 natural molecules. The goal of this study was to expand on earlier studies showing the beneficial effects of raw pyrolysis bio-oil on regulating IL-8 levels in keratinocytes via inhibiting IL-17. The investigation started by examining the side effects and pharmacological interactions of traditional herbal therapies for psoriasis. The bio-oil fractionation procedure was then revised and improved in the research to produce five different bio-oil fractions, each of which was characterized and put to the test on keratinocytes stimulated with IL-17. The most consistent anti-IL-17 action was seen in the aqueous fraction, which contained three pure compounds. The levels of IL-8 were effectively modulated by the mixture of two of these compounds, namely, acetic acid and acetol. According to RNA-Seq data, mitogen-activated protein kinase (MAPK) activity was found to be negatively regulated by 29 genes. This study was successful in locating effective anti-IL-17 molecules and offering details on their potential mechanisms of action.
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Details
- Title
- Fractionation and Characterization of a Pine Pyrolysis Bio-Oil and In Vivo and In Silico Analysis of Its Anti-IL-17 Effects on HaCaT Human Keratinocytes
- Creators
- Marwan Gagaa
- Contributors
- Manuel Garcia-Perez (Advisor)Shulin Chen (Committee Member)Sindhu Sankaran (Committee Member)Stephen Ficklin (Committee Member)John Hinz (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Biological Systems Engineering
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 165
- Identifiers
- 99901086723301842
- Language
- English
- Resource Type
- Dissertation