Dissertation
Fundamentals and applications of high resolution electrospray ion mobility spectrometry
Washington State University
Doctor of Philosophy (PhD), Washington State University
12/1999
DOI:
https://doi.org/10.7273/000006220
Abstract
Resolving powers as high as 150 for singly charged ions has been achieved for ion mobility spectrometry. This increased resolving power resulted from a few modifications to an existing ion mobility spectrometer interfaced to a mass spectrometer. This arrangement allowed the separation of leucine and isoleucine in the gas phase in less than 30ms. In addition to increasing the resolving power, the ultimate resolution in IMS was improved by using multiple drift gases. The polarizability of the drift gases has a dramatic effect on ions as they drift down the tube. The magnitude of these effects was ion dependant and showed significant differences for different ions. These differences can be exploited in IMS to separate ions in one drift gas that may not be resolved in another. Using calculations that describe ion transport through gases and assuming hard spheres for both the ions and the neutral buffer gas molecules, a linear correlation was obtained between the calculated ion radius and the polarizability of the drift gas. This linear relationship can be used to determine the radius of an ion in a non-polarizing gas. This strategy proved useful in measuring ion size in the gas phase by IMS and comparing these measured value to those made by using a computer modeling program, CHARMn. Finally IMS was shown to have the potential to be a powerful analytical separation tool. Three common explosives: TNT, RDX, and HMX were separated in less than 25ms. In addition to explosives, four common chemical warfare (CW) degradation products from G-type nerve gas were separated in less than 20ms. These same four compounds were separated in a river water sample demonstrating the ability of the technique to handle environmental samples. Finally it was shown that the 20 common amino acids could be distinguished by IMS. This demonstrated the possibility of using IMS as a very rapid, sensitive amino acid analyzer.
Metrics
1 File views/ downloads
19 Record Views
Details
- Title
- Fundamentals and applications of high resolution electrospray ion mobility spectrometry
- Creators
- G. Reid Asbury
- Contributors
- Herbert H. Hill Jr. (Chair) - Washington State University, Department of ChemistryWilliam F Siems (Committee Member) - Washington State University, Department of ChemistryChing Wu (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Chemistry
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 212
- Identifiers
- 99901080641301842
- Language
- English
- Resource Type
- Dissertation