Dissertation
Introgressing multiple disease resistance alleles into elite apple cultivars by DNA-informed breeding
Doctor of Philosophy (PhD), Washington State University
01/2019
Handle:
https://hdl.handle.net/2376/111177
Abstract
Major apple diseases cause significant damage to apple production worldwide because highly susceptible cultivars are widely planted, particularly for apple foliar mildew, fire blight, and blue mold. Diseases losses can be minimized if cultivars with durable resistance are planted. Most sources of resistance are apple wild relatives that usually have poor fruit quality. Developing cultivars with durable resistance combined with required elite fruit quality phenotypically is difficult and time-consuming. DNA markers would be useful for efficiently identifying suitable offspring by the detection of disease resistance alleles and genome-wide tracking of DNA segments from apple wild relatives. The objectives of this project were to: 1) develop DNA tests for apple foliar mildew (source: ‘White Angel’), fire blight (source: ‘Enterprise’), and blue mold resistance (source: Malus sieversii PI 613981); and 2) demonstrate efficient introgression for blue mold resistance. For creating new DNA tests, four families used were ‘Fuji’ × ‘White Angel’ (n = 102) and ‘Golden Delicious’ × ‘White Angel’ (n = 92) for mildew and ‘Enterprise’ × T1190 (n = 219) for fire blight, and ‘Gala’ × PI 613981 (n = 89) for blue mold. For introgression of blue mold resistance, the families used were GMAL4593-128 and -175 [‘Gala’ × PI 613981] × T1190 [BpMADS4-transgenic ‘Pinova’ × ‘Idared’] (n = 141). Apple 20K SNP array data was used to trace the inheritance of M. sieversii DNA segments. Three locus-specific DNA tests, Md-Plw8-SSR, Md-Ea7-SSR, and Md-Pe3-SSR, were successfully developed, which were able to distinguish susceptible individuals from resistant ones. These DNA tests themselves or combined with other disease resistance DNA tests could be used to efficiently detect the presence of resistance alleles from multiple sources. Within two years, among greenhouse-grown 141 individuals, three were selected with the resistance allele, the least proportion of M. sieversii DNA segments, and favorable positions of these segments on the same chromosome as the resistance allele. The combined use of a locus-specific DNA test, high-density SNP array data, and rapid cycle breeding was successfully demonstrated. Outcomes of this project could empower the development of new apple cultivars with durable resistance and elite fruit quality.
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Details
- Title
- Introgressing multiple disease resistance alleles into elite apple cultivars by DNA-informed breeding
- Creators
- Feixiong Luo
- Contributors
- Peace Cameron (Chair)John Norelli (Committee Member)Kate Evans (Committee Member)Zhiwu Zhang (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Horticulture
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 195
- Identifiers
- 99900581817501842
- Language
- English
- Resource Type
- Dissertation