Files and links (9)
CC BY-NC-SA V4.0, Open Access
ImageCC BY-NC-SA V4.0, Open Access
ImageCC BY-NC-SA V4.0, Open Access
SpreadsheetCC BY-NC-SA V4.0, Open Access
SpreadsheetCC BY-NC-SA V4.0, Open Access
Dissertation
Linking epidemiologically distinct genotypes to phenotypes of Salmonella Kentucky
Washington State University
Doctor of Philosophy (PhD), Washington State University
2022
DOI:
https://doi.org/10.7273/000005015
Abstract
Salmonella Kentucky is an emerging foodborne pathogen comprised of two major genetic lineages, sequence types (ST)198 and ST152. S. Kentucky ST152 is prevalent in US poultry and sporadically associated with human disease while ST198 is prevalent in poultry outside of the US and commonly associated with human disease worldwide. It is unknown why ST198 is associated with an increased incidence of human disease. Here, we hypothesized that ST198 and ST152 are epidemiologically and genetically distinct and display differential metabolism and virulence. We performed genetic characterization of S. Kentucky isolated from human patients in Washington State, compared these with S. Kentucky isolated from poultry and other food animal sources, and attributed them to unique epidemiological sources. We then identified lineage-specific genetic differences that clearly distinguish these two lineages. Using representative isolates from each of the two major genetic lineages, we identified unique metabolic phenotypes that clearly distinguish these two lineages as metabolically distinct. The results of this study show that ST198 and ST152 lineages of S. Kentucky have metabolically diverged. When compared with ST152, ST198 is significantly more efficient in its ability to utilize a constellation of carbon compounds as sole energy sources for survival and growth, including 1,2-propanediol and m-inositol. The metabolic fitness of ST198 likely contributes to the nutritional virulence potential of ST198, leading to its global emergence. The metabolic phenotypes identified in this study may serve as a tool for studying the evolutionary trajectories of ST198 and ST152 and the differential virulence of these two lineages. These data may also aid in epidemiologic source tracing the ST198 and ST152 lineages, and potentially serve as targets for the development of therapeutics and prophylactics.
Metrics
6 File views/ downloads
26 Record Views
Details
- Title
- Linking epidemiologically distinct genotypes to phenotypes of Salmonella Kentucky
- Creators
- Rachel Christine Soltys
- Contributors
- Devendra H. Shah (Advisor)Kelly A. Brayton (Committee Member)Omar Cornejo (Committee Member)Bradd J. Haley (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- College of Veterinary Medicine
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 63
- Identifiers
- 99901019237601842
- Language
- English
- Resource Type
- Dissertation