Dissertation
MODELING TEMPERATURE DEPENDENT DYNAMIC CONTRACTILE PROPERTIES IN HUMAN SKELETAL MUSCLE FIBERS USING CROSSBRIDGE MODELS
Doctor of Philosophy (PhD), Washington State University
01/2011
Handle:
https://hdl.handle.net/2376/2843
Abstract
The goal of mathematical muscle models is to predict the force-generating properties during <italic>in vivo</italic> contractions. The development of such models necessitates accurate measurements of the mechanical properties from whole individual muscles at <italic>in vivo</italic> conditions. However, it is very difficult to isolate any specific muscle to measure its precise mechanical properties, especially in humans. One approach to addressing this problem is to isolate individual muscle fibers, measure their mechanical properties and integrate these data into muscle models to simulate their <italic>in vivo</italic> properties. Because each individual muscle is a heterogeneous mixture of slow (type I) and fast (type II) fiber populations, it is also necessary to identify the specific mechanical contributions from each fiber type population. A major challenge in this approach is that because of loss of structural integrity during <italic>in vitro</italic> fiber measurements at physiological temperatures (~37 °C), isolated muscle fibers can only be tested at lower temperatures (<30 °C). This information can still be valuable to identify the temperature-dependency of the contractile kinetics in the lower temperature range and to extend this dependency to predict the kinetic parameters at the <italic>in vivo</italic> temperature condition via extrapolation. <bold>Therefore, the overall objective of this study is to estimate the in vivo properties of muscle fibers based on mechanical measurements at lower temperatures.</bold> This research will specifically focus on:
1. Measurement of the static Force-Length relationship from isolated human type I fibers.
2. Measurements of the Force-Velocity relationships during both shortening and lengthening at various temperatures (15-30 °C) from isolated human type I fibers. The Force-Length and Force-Velocity properties will be integrated into a two-state crossbridge model to estimate the model parameters and to validate whether this model can simulate the temperature-dependent fiber behavior reasonably well toward estimating <italic>in vivo</italic> properties.
3. A three-state model that may more accurately simulate the type I muscle fiber properties and the temperature-dependent relationships of each model parameter are identified. The <italic>in vivo</italic> muscle fiber properties (37 °C) will be predicted from these relationships.
Metrics
Details
- Title
- MODELING TEMPERATURE DEPENDENT DYNAMIC CONTRACTILE PROPERTIES IN HUMAN SKELETAL MUSCLE FIBERS USING CROSSBRIDGE MODELS
- Creators
- Sampath Gollapudi
- Contributors
- David C Lin (Advisor)Anita Vasavada (Committee Member)Jow-Lian Ding (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Mechanical and Materials Engineering, School of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 135
- Identifiers
- 99900581460701842
- Language
- English
- Resource Type
- Dissertation