Dissertation
NOVEL FLUORESCENT SENSORS AND SCAVENGERS OF HYDROGEN SULFIDE
Washington State University
Doctor of Philosophy (PhD), Washington State University
01/2021
DOI:
https://doi.org/10.7273/000005418
Handle:
https://hdl.handle.net/2376/119379
Abstract
Owing to its unique regulatory effects in many physiological and pathological processes, the study of hydrogen sulfide (H2S) has attracted considerable attention in the past decade. Precise evaluation and regulation of H2S are critical to understand the exact action mechanism and clinical potentials of H2S in biological matrix. However, as a reactive gaseous molecule, H2S quickly degrades in aqueous and biological samples due to volatilization and fast biochemical reactions. The knowledge of accurate H2S concentrations needed for protective effects are still lacking. This presents a major burden in the development of H2S therapy, especially for the use of synthetic H2S regulating compounds. In this regard, novel selenenyl-sulfide based fluorescent probes to achieve accurate and non-destructive H2S measurement in biological samples were developed. Sulfonyl azides as specific H2S scavengers were discovered, the efficiency of these scavengers in in vitro and in vivo experiments were demonstrated. A database of xanthene dyes was constructed, a series of new xanthene dyes by applying deep learning technology were discovered. These compounds serve as important chemical tools for further investigating chemistry and therapeutic potentials of H2S.
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Details
- Title
- NOVEL FLUORESCENT SENSORS AND SCAVENGERS OF HYDROGEN SULFIDE
- Creators
- Yingying Wang
- Contributors
- Ming Xian (Advisor)Cliff E. Berkman (Advisor)Greg Crouch (Committee Member)Qiang Zhang (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Chemistry
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 207
- Identifiers
- 99900592360801842
- Language
- English
- Resource Type
- Dissertation