Dissertation
New chemistry of S-nitrosothiols
Doctor of Philosophy (PhD), Washington State University
01/2012
Handle:
https://hdl.handle.net/2376/4060
Abstract
Protein S-nitrosation is an important post-translational modification that not only transmits nitric oxide (NO) biological activity, but also regulates protein functions. However, development of reliable methods for the detection of protein S-nitrosation is still a challenge due to the lability of its products S-nitrosothiols (SNOs).
The thesis will discuss three aspects in detail: first, the development of fluorescent probes for the detection of SNOs based on the reductive ligation; second, the study on the reactivity of sulfenamides which is the reductive ligation product from SNOs, towards different nucleophiles, and further development of a one-pot disulfide formation reaction; lastly, the synthetic application of S-nitrosation in amide/peptide synthesis.
The new chemistry of S-nitrosothiols not only provides a promising platform for the study of S-nitrosation in biological system, but also reveals the potential usage of S-nitrosation in organic synthesis.
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Details
- Title
- New chemistry of S-nitrosothiols
- Creators
- Jia Pan
- Contributors
- Ming Xian (Advisor)Cliff Berkman (Committee Member)Phil Garner (Committee Member)Rob Ronald (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Chemistry, Department of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 230
- Identifiers
- 99900581456301842
- Language
- English
- Resource Type
- Dissertation