Dissertation
Redefining cyclic electron flow around photosystem I (CEF1): The induction, pathway, and role of CEF1 in C3 plants
Washington State University
Doctor of Philosophy (PhD), Washington State University
05/2010
DOI:
https://doi.org/10.7273/000006136
Abstract
While traditional photosynthetic research has focused on the "linear" electron transfer pathway, alternative "cyclic" pathways have been proposed as a means to balance energy needs in plants. After decades of work, spanning a diverse field of techniques and ideas, controversy remains as to the pathway, role and regulation of cyclic electron flow around photosystem I (CEF1). CEF1 must be elucidated to understand how plants respond to and survive changing environmental stresses, such as drought, cold, heat and salt. We have isolated a new mutant phenotype where CEF1 is greatly increased with respect to normal photosynthesis or linear electron transfer. These high CEF1, or hcef, mutants provide a unique opportunity for answering key questions about the regulation, role, and pathway of CEF1. Through the utilization of map-based cloning, new spectroscopic techniques, and crossing with other known CEF1 mutants, we have determined that CEF1 is a highly dynamic, regulated, and large capacity pathway in plants. CEF1 in C3 plants appears to run through the thylakoid NAD(P)H dehydrogenase (NDH) complex and not the once favored antimycin A-sensitive ferredoxin-plastoquinone oxidoreductase (FQR), or PGR5 (proton gradient regulator 5) dependent pathway. Furthermore, hydrogen peroxide has been found to be both an inducer of the formation of the NDH complex and activator of NDH mediated CEF1.
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Details
- Title
- Redefining cyclic electron flow around photosystem I (CEF1)
- Creators
- Aaron Kyle Livingston
- Contributors
- David M. Kramer (Chair)William B Davis (Committee Member) - Washington State University, School of Molecular BiosciencesAmit Dhingra (Committee Member) - Washington State University, Department of HorticultureGerald E Edwards (Committee Member) - Washington State University, School of Biological SciencesAndris Kleinhofs (Committee Member) - Washington State University, Department of Crop and Soil Sciences
- Awarding Institution
- Washington State University
- Academic Unit
- School of Molecular Biosciences
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 165
- Identifiers
- 99901055020101842
- Language
- English
- Resource Type
- Dissertation