Dissertation
SILICA NANOSTRUCTURE PLATFORM FOR AFFINITY CAPTURE OF TUMOR-DERIVED EXOSOMES AND OTHER BIOMEDICAL APPLICATIONS OF NANOMATERIALS
Doctor of Philosophy (PhD), Washington State University
01/2017
Handle:
https://hdl.handle.net/2376/111513
Abstract
The integration of nanotechnology with medicine has revolutionized the twentieth century and can be developed into categories of diagnostic devices, contrast agents, sensors, physical therapy applications, and drug delivery vehicles. My research has focused mostly on the development of a diagnostic technique to monitor the progression of prostate cancer and early diagnosis of cancer.
Biofunctionalized silica nanospring mats were prepared to selectivity capture tumor-derived exosomes through the interaction of the ligand with prostate-specific membrane antigen on the exosomes. The result obtained from biocinchonic acid assay shows high capture efficiency for the ligand-activated silica nanostructures. The silica nanostructures provide a high surface area support for immobilization of biomolecules and the open structure of the mats improves the accessibility of the flowing solvent. Tumor-derived exosomes are secreted by cancerous known as a new resource for the biomarker to facilitate early diagnosis via a non-invasive procedure. This proposed work is expected to accelerate the discovery of diagnostic or prognostic biomarkers.
Another application of nanotechnology has been investigated in chapter 4 and 5. The potential of a nanoparticle delivery system has been evaluated to improve methods of delivery of candidate peptide-based vaccines for paratuberculosis. Poly (lactic-co-glycolic acid) nanoparticles vector containing the peptide elicit the development of cytotoxic T cells with the ability to kill intracellular mycobacterium avium ssp. Paratuberculosis which is the causative agent of Johne’s disease. The pathogen has a broad host range that includes ruminants and non-ruminant species, including humans. Because Johne’s disease is so difficult to diagnose before it progresses to clinical disease, vaccination is the only viable approach for safeguarding cattle and humans from the dangerous effects of paratuberculosis.
Metrics
5 File views/ downloads
27 Record Views
Details
- Title
- SILICA NANOSTRUCTURE PLATFORM FOR AFFINITY CAPTURE OF TUMOR-DERIVED EXOSOMES AND OTHER BIOMEDICAL APPLICATIONS OF NANOMATERIALS
- Creators
- Parissa Ziaei
- Contributors
- M.Grant Norton (Advisor)Clifford E Berkman (Committee Member)Ursula Mazur (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Materials Science and Engineering Program
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 221
- Identifiers
- 99900581426701842
- Language
- English
- Resource Type
- Dissertation