Dissertation
SURROGATE SIRES: DONOR-DERIVED SPERMATOGENESIS IN NANOS2 KNOCKOUT MICE AND LIVESTOCK VIA SPERMATOGONIAL STEM CELL TRANSPLANTATION
Doctor of Philosophy (PhD), Washington State University
01/2020
Handle:
https://hdl.handle.net/2376/111142
Abstract
Spermatogonial stem cell transplantation (SSCT) is an experimental technique for transfer of germline between donor and recipient males that has important application as a tool in biomedical research, dissemination of desirable genetics in food animal populations, and preservation of endangered species. To realize these potentials, recipient males that are devoid of endogenous germline but possess normal testicular architecture and somatic cell function that can support allogeneic donor stem cell engraftment and regeneration of spermatogenesis are essential. Here, we show that male mice and pigs rendered germline ablated by CRISPR-Cas9 knockout of the NANOS2 gene are effective SSCT recipients. With mice, recipients attain natural fertility when subjected to SSCT with allogeneic donor stem cells during prepubertal development. For recipient pigs and goats, SSCT with allogeneic donor stem cells in advanced adulthood led to sustained donor-derived spermatogenesis. Following refinements, SSCT with NANOS2 knockout recipient males to generate surrogate sires may be at the brink of impactful application as a breeding tool in biomedical research and livestock production.
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Details
- Title
- SURROGATE SIRES: DONOR-DERIVED SPERMATOGENESIS IN NANOS2 KNOCKOUT MICE AND LIVESTOCK VIA SPERMATOGONIAL STEM CELL TRANSPLANTATION
- Creators
- Michela Ciccarelli
- Contributors
- Jon M Oatley (Advisor)Ahmed Tibary (Committee Member)Michael D Griswold (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 143
- Identifiers
- 99900581499401842
- Language
- English
- Resource Type
- Dissertation