Dissertation
SYNTHESIS, STRUCTURE AND APPLICATIONS OF NOVEL RHODAMINE DERIVATIVES
Doctor of Philosophy (PhD), Washington State University
01/2016
Handle:
https://hdl.handle.net/2376/111319
Abstract
Recently, fluorescence imaging microscopy has undergone through revolutionary advancement in spatial resolution, improving from submicron meter resolution to nanometer resolution that rivals those of electron microscopes. The key enabling elements of the innovative super-resolution fluorescence microscopy are the photoswitchable fluorophores. In this thesis, a diverse set of photoswitchable rhodamine fluorophores have been synthesized by varying the intra- and inter-molecular substitution on the spiro carbon. The desired photoswitching property enabled nanostructural determination, and indeed nanostructures in biological cells were successfully resolved beyond the limit of optical diffraction. While analogous to photochromism, thermochromism of synthesized rhodamine derivatives was also studied and employed as a means to encode information into optical fibers. Interestingly, when an orthogonal pair of a photochromic dye and a thermochromic dye was used, orthogonal stimuli of light and heat impart information encoding simultaneously and independently, thus realizing multiplexing in data encoding and transmission. To understand the unusual structures of photoswitches and thermoswitches, we developed a general procedure to grow single crystals and used X-ray crystallography to study their structure-property relationships.
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Details
- Title
- SYNTHESIS, STRUCTURE AND APPLICATIONS OF NOVEL RHODAMINE DERIVATIVES
- Creators
- Baoming Zhao
- Contributors
- Alexander D. Q. Li (Advisor)Clifford E. Berkman (Committee Member)Zachariah M. Heiden (Committee Member)Jeanne L. McHale (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Chemistry
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 224
- Identifiers
- 99900581830301842
- Language
- English
- Resource Type
- Dissertation