Dissertation
TEMPERATURE AND GENETIC EFFECTS ON GROWTH, BODY SHAPE, AND SPRINT SWIM SPEED IN RAINBOW TROUT
Washington State University
Doctor of Philosophy (PhD), Washington State University
01/2021
DOI:
https://doi.org/10.7273/000003133
Handle:
https://hdl.handle.net/2376/122257
Abstract
Ultimate phenotype for a given trait results from the combined influences of underlying genetics and environmental factors; predicting how organisms respond to changing environments thus depends on understanding the relative impacts of genes and environmental variables on phenotypes. Here, I explore how changes in one of the most fundamental environmental variables, temperature, impact phenotypes with strong survival implications for rainbow trout (Oncorhynchus mykiss), and explore the underlying genetic architecture associated with phenotypic change in response to temperature. In this dissertation, I begin by comparing responses of growth, size, body shape, and sprint swimming speed phenotypes to rearing at 10o C and 18o C in two geographically disparate, semi-wild clonal strains of O. mykiss (Whale Rock clonal line, from California, and Swanson clonal line, from Alaska), compared to two clonal strains with hatchery origins (Arlee and Hot Creek clonal lines). I find that Whale Rock and Swanson overall show the greatest between-line differences in these phenotypes, and vary notably in the nature and magnitude of their plastic response to temperature. I then explore the influence of 10o C and 18o C rearing temperature on the location of body fins that potentially influence sprint swimming speeds (dorsal fin, anal fin, and pelvic fins) at 32 and 35 weeks of age in Whale Rock and Swanson clonal lines, with Hot Creek as a hatchery-origin clonal line for comparison. I find complex patterns of variation in fin location at both ages, both within clonal line at different temperatures and between clonal lines within the same temperature. Of the fin locations measured, only one was found to significantly influence sprint swimming speed, which was the distance between the operculum and the most anterior margin of the anal fin. Whale Rock and Swanson showed distinctly disparate differences in fin metrics, again suggesting their suitability for exploring genetic architecture of these phenotypes. Finally, I use a quantitative genetic approach to identify quantitative trait loci (QTL), finding evidence for significant and suggestive QTL influencing the measured phenotypes, as well as evidence for temperature-specific QTL.
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Details
- Title
- TEMPERATURE AND GENETIC EFFECTS ON GROWTH, BODY SHAPE, AND SPRINT SWIM SPEED IN RAINBOW TROUT
- Creators
- Christopher Steven Duke
- Contributors
- Patrick A Carter (Advisor)Paul A Hohenlohe (Committee Member)Brian P Kennedy (Committee Member)Gary H Thorgaard (Committee Member)Heather E Watts (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Biological Sciences, School of
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 241
- Identifiers
- 99900651900101842
- Language
- English
- Resource Type
- Dissertation