Dissertation
The role of the RNA binding protein OsTudor-SN during rice grain development as viewed by transcriptome analysis
Washington State University
Doctor of Philosophy (PhD), Washington State University
01/2021
DOI:
https://doi.org/10.7273/000006448
Handle:
https://hdl.handle.net/2376/119402
Abstract
Tudor-SN is a conserved RNA binding protein in eukaryotes. The Tudor-SN consists of two structures, the 4SN module and the Tsn module. The 4SN module consists of four staphylococcus nuclease (SN) domains located at the N-terminal region. The Tsn module is composed of a Tudor domain and a partial SN domain located at the C-terminal side. A recent study revealed that these two modules of OsTudor-SN have different binding properties. The 4SN module has RNA and protein binding activities, while the Tsn module has protein binding activity. Although both modules bind to proteins, they bind to different groups. These observations point to the possibility that the 4SN and the Tsn modules may have biological significance on their own. To determine whether the two modules have distinct roles in governing gene expression, the developing seed transcriptomes of OsTudor-SN mutant lines, EM1084, 43M and PMT64 and wildtype were obtained and analyzed. Compared to wildtype there are 1865, 1450, and 954 differentially expressed genes in 43M, EM1084, and PMT64, respectively. Among these, 78 genes are present in all OsTudor-SN mutant lines. The expression of storage protein mRNAs in the three mutant lines were not identical. 43M and PMT64 showed elevated expression of cysteine-rich prolamins while EM1084 displayed decreased expression of cysteine poor prolamins. Many genes involved in translation, especially those coding for ribosomal proteins were elevated in 43M. The mutation at the SN3 in EM1084 altered the expression of genes involved in carbohydrate metabolic process and membrane vesicle-mediated transport. The mutation at the Tudor domain in PMT64 mainly affects carbohydrate metabolic process.
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Details
- Title
- The role of the RNA binding protein OsTudor-SN during rice grain development as viewed by transcriptome analysis
- Creators
- Maiporn Maipoka
- Contributors
- Thomas Okita (Advisor)Andrew McCubbin (Committee Member)John Wyrick (Committee Member)Patricia Okubara (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Program in Molecular Plant Sciences
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Publisher
- Washington State University
- Number of pages
- 131
- Identifiers
- 99900592361901842
- Language
- English
- Resource Type
- Dissertation