Dissertation
VARIATION IN PHOTOSYNTHETIC ENZYME KINETIC AND CARBON ISOTOPIC FRACTIONATION PARAMETERS BETWEEN TEMPERATURES
Doctor of Philosophy (PhD), Washington State University
01/2017
Handle:
https://hdl.handle.net/2376/108470
Abstract
The photosynthetic assimilation of carbon dioxide in C3 plants is potentially limited by the enzymatic rates of Rubisco, and in C4 plants additional limitations are imposed by phosphoenolpyruvate carboxylase, and carbonic anhydrase. Therefore, the activity and kinetic properties of these enzymes are needed to accurately parameterize C3 and C4 biochemical models of leaf carbon dioxide exchange in response to changes in carbon dioxide availability and temperature. Here, we used membrane inlet mass spectrometry to measure the temperature responses of Rubisco carboxylation and oxygenation kinetics, phosphoenolpyruvate carboxylase carboxylation kinetics, and the activity and first-order rate constant for carbonic anhydrase hydration reaction from ten to forty degree Celsius. Additionally, comparisons of the Rubisco temperature response determined by membrane inlet mass spectrometry are compared to traditional methods following the consumption of radiolabeled substrate. Special attention is paid to the possibility of breakpoints in the Rubisco temperature response. Finally, the temperature response of Rubisco discrimination of thirteen-carbon-carbon dioxide is presented.
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Details
- Title
- VARIATION IN PHOTOSYNTHETIC ENZYME KINETIC AND CARBON ISOTOPIC FRACTIONATION PARAMETERS BETWEEN TEMPERATURES
- Creators
- Ryan Allen Boyd
- Contributors
- Asaph B Cousins (Advisor)Gerald E Edwards (Committee Member)Eric H Roalson (Committee Member)Sanja Roje (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Program in Molecular Plant Sciences
- Theses and Dissertations
- Doctor of Philosophy (PhD), Washington State University
- Number of pages
- 163
- Identifiers
- 99900581516301842
- Language
- English
- Resource Type
- Dissertation