Essay
Effects of relative humidity and applied force on atomic force microscopy images of the S-form of phage fd
1999
Handle:
https://hdl.handle.net/2376/2538
Abstract
The filamentous phage fd was converted to spheroidal shaped S-forms and studied by contact-mode atomic force microscopy under conditions of controlled differences in relative humidity coupled with changes in the applied tip force. Stable and reliable sample preparation was achieved by spin-coating freshly cleaved mica with particle containing solutions with very low salt content followed by humidity control. The filaments of fd form a plateau and then drop in height as increased force is applied [15]. The apparent height, however, of the S-forms decreased linearly as force increased, due to their nonrigid side chain packing. The findings that relative humidity plays a role on reliable and reproducible images is consistent with previously published results on other biological specimens.
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Details
- Title
- Effects of relative humidity and applied force on atomic force microscopy images of the S-form of phage fd
- Creators
- Catherine Ratliff (Author)
- Academic Unit
- Honors Theses (WSU Pullman, Passed with Distinction)
- Identifiers
- 99900590744601842
- Copyright
- http://rightsstatements.org/vocab/InC/1.0/; http://www.ndltd.org/standards/metadata; http://purl.org/eprint/accessRights/OpenAccess; In copyright; Publicly accessible; openAccess
- Language
- English
- Resource Type
- Essay