Essay
Whisker stimulation increases the number of tumor necrosis factor α- immunoreactive cells in the primary somatosensory cortex
2006
Handle:
https://hdl.handle.net/2376/2456
Abstract
Tumor necrosis factor alpha (TNFa) is a cytokine that plays an important role in neural plasticity as well as in the regulation of sleep. Immunohistochemistry was used to locate and analyze TNFa- and Fos-immunoreactive (lR) cells in brain sections taken from animals receiving 2 hours of whisker deflection. In layer IV (the layer that receives the primary sensory input to the cortex) as well as in layers II-III (layers involved in modulation of the sensory processing), the number of TNFa-IR cells increased in the cortical columns that showed Fos activation in an adjacent section. In another experiment where an individual whisker was twitched and the physiological response in the cortex was recorded, the number ofTNFa-IR cells increased significantly in layer IV beneath the microelectrodes that recorded the physiological response. Double labeling with fluorescent probes demonstrated that Fos-IR nuclei were present in TNFa-IR cells and that TNFa was present in neurons selectively labeled with a neuro arker, NeuN. Collectively these data suggest that afferent activation of a cortical column enhances TNFa production in neurons providing support for the hypothesis that sleep is initiated within local networks and is activity-dependent. 9
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Details
- Title
- Whisker stimulation increases the number of tumor necrosis factor α- immunoreactive cells in the primary somatosensory cortex
- Creators
- Cora Fix (Author)
- Contributors
- Lynn Churchill (Advisor)
- Academic Unit
- Honors Theses (WSU Pullman, Passed with Distinction)
- Identifiers
- 99900590551501842
- Copyright
- http://rightsstatements.org/vocab/InC/1.0/; http://www.ndltd.org/standards/metadata; http://purl.org/eprint/accessRights/OpenAccess; In copyright; Publicly accessible; openAccess
- Language
- English
- Resource Type
- Essay