Thesis
A marker for the Ug99 stem rust resistance gene Sr28 in spring wheat and evaluation of wheat stripe rust susceptibility in Brachypodium distachyon
Washington State University
Master of Science (MS), Washington State University
2015
Handle:
https://hdl.handle.net/2376/103199
Abstract
Wheat is one of the most widely grown crops in the world and is threatened every year by pathogens in the genus Puccinia. The use of resistant cultivars is the most economical, and effective method of protecting against yield losses due to Puccinia rust diseases of wheat. The TTKSK (Ug99) race group of wheat stem rust (Puccinia graminis f.sp. tritici) is highly virulent against most resistance genes used globally in wheat cultivars. The stem rust resistance gene Sr28 demonstrates resistance against Ug99, and if it can be integrated with other resistance genes it may help create durable resistance against this new race group that is spreading across Africa and Asia. The marker STS 7975441-N developed through this project demonstrates linkage with this gene and can help wheat breeders integrate Sr28 into elite wheat cultivars. Another species of rust, Puccinia striiformis f. sp. tritici, known commonly as stripe rust is one of the most economically damaging diseases of wheat worldwide. The model organism Brachypodium distachyon may be able to provide insight into wheat resistance mechanisms, if a wheat stripe rust susceptible line of the grass species can be developed. Towards creating a stripe rust susceptible line of B. distachyon preliminary work herein screened available germplasm for P. striiformis f. sp. tritici infection. In Washington stripe rust screening nurseries, twelve B. distachyon lines showed substantial uredinia. A subset of these partially susceptible lines were crossed to each other in hopes of obtaining more susceptible progeny, and one of these lines was mutagenized towards the same goal. The resulting plant materials are still being screened, however preliminary results indicate the M2s and F2s have abundant sporulation.
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Details
- Title
- A marker for the Ug99 stem rust resistance gene Sr28 in spring wheat and evaluation of wheat stripe rust susceptibility in Brachypodium distachyon
- Creators
- Elizabeth W. Elmore
- Contributors
- Michael O. Pumphrey (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Crop and Soil Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525083601842
- Language
- English
- Resource Type
- Thesis